Further Studies on the Labile Flavin Adenine Dinucleotide of Heart Muscle Preparations

IT was observed by Massey and Swoboda 1 that on storage at 4° of Keilin–Hartree preparations isolated by centrifugation at p H 5.4 a gradual breakdown of FAD to FMN occurs. Immediately after isolation such preparations contained up to 95 per cent of the acid-extractable flavins in the form of FAD, b...

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Bibliographic Details
Published in:Nature (London) 1964-03, Vol.201 (4923), p.1027-1028
Main Authors: SALACH, JAMES, KEARNEY, EDNA B, SINGER, THOMAS P
Format: Article
Language:English
Subjects:
Flavin Mononucleotide
Flavin-Adenine Dinucleotide
Humanities and Social Sciences
Hydrogen-Ion Concentration
letter
multidisciplinary
Myocardium
NAD
Old Medline
Pyrophosphatases
Refrigeration
Science
Science (multidisciplinary)
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Summary:IT was observed by Massey and Swoboda 1 that on storage at 4° of Keilin–Hartree preparations isolated by centrifugation at p H 5.4 a gradual breakdown of FAD to FMN occurs. Immediately after isolation such preparations contained up to 95 per cent of the acid-extractable flavins in the form of FAD, but this quantity decreased to about 70 per cent over a period of several days. These authors concluded that in strictly fresh Keilin–Hartree preparations and in mitochondria, within experimental error, all the acid-extractable flavin may be in the form of FAD, and suggested, therefore, that FMN enzymes isolated from these sources might be artefacts.
ISSN:0028-0836
1476-4687
DOI:10.1038/2011027a0