Shear and Pearse Method for Direct Histochemical Demonstration of Sialic Acid

A DIRECT histochemical method for the demonstration of sialic acid was recently published by Shear and Pearse 1 which is based on a method described by Svennerholm 2 . The procedure as described by Shear and Pearse requires that the reaction be carried out on fresh-frozen cryostat sections (8µ), mou...

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Bibliographic Details
Published in:Nature (London) 1964-02, Vol.201 (4919), p.630-630
Main Authors: ZUGIBE, FREDERICK T, PEPINE, CARL, BELL, PAUL, MUST, JAMES
Format: Article
Language:English
Subjects:
Animals
Aorta
Formaldehyde
Histocytochemistry
Humanities and Social Sciences
letter
multidisciplinary
N-Acetylneuraminic Acid
Neuraminic Acids
Neuraminidase
Old Medline
Rats
Science
Science (multidisciplinary)
Submandibular Gland
Swine
Trachea
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Summary:A DIRECT histochemical method for the demonstration of sialic acid was recently published by Shear and Pearse 1 which is based on a method described by Svennerholm 2 . The procedure as described by Shear and Pearse requires that the reaction be carried out on fresh-frozen cryostat sections (8µ), mounted on albumin-coated glass slides which have been exposed to formaldehyde vapour in a closed desiccator for at least 72 h and washed in running water for 2 min and allowed to dry. The reaction is then carried out by covering the sections with a few drops of resorcinol hydrochloride. After 2 min, excess reagent is removed and the sections treated with a few drops of 1 per cent sodium nitrite in concentrated hydrochloric acid. The red colour which develops is attributed to sialic acid sites and the intensity proportioned to the sialic acid concentration.
ISSN:0028-0836
1476-4687
DOI:10.1038/201630a0