Propagation of Mouse Adenovirus on Cell Lines of Human Origin

• Published in: Nature (London) 1964-06, Vol.202 (4937), p.1139-1140
• Main Authors: SHARON, NEHAMA, POLLARD, MORRIS
• Format: Article
• Language: English
• Subjects: Adenoviridae; Carcinoma, Squamous Cell; Cell Line; Fibroblasts; Humanities and Social Sciences; Inclusion Bodies; Kidney; letter; Mice; multidisciplinary; Old Medline; Science; Science (multidisciplinary); Synovial Membrane; Tissue Culture Techniques
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/2021139a0

SOME common properties described for adenovirus strains are: (1) distinct intranuclear inclusion bodies in infected cells; (2) grape-like pattern of cytopathogenic effect; (3) group complement-fixing antigen; (4) uniform hexagonal ultrastructure; (5) a “relative species specific host range in animals and in tissue cultures” as described by Howe and Hartley 1 . Strains of human adenoviruses induced disease in the natural host or in closely related genera, and the titres of virus were higher in human cells. Human adenoviruses have been propagated in non-human tissues: Betts et al. 2 demonstrated infection in pathogen-free piglets with human adenovirus, Type 1; and the oncogenic effect of human adenovirus 12 and 18 was demonstrated by Trentin et al. 3 and by Huebner et al. 4 in hamsters. In addition, human adenovirus has been propagated in tissues of various animals: Guerin and Guerin 5 in pig kidneys; Rowe et al. 6 in rabbit trachea and cotton rat tissues; Kelly and Pereira 7 in rabbit kidneys; and Warren and Cutchins 8 in bovine and embryonic tissue culture. In regard to propagation of animal adenoviruses it has been stated by Ginsberg 9 that “adenovirus of animal origin propagates best and initiates distinctive cyto-pathology in tissues from homologous species”, and in another report Rowe and Hartley 1 stated that mouse and chicken adenoviruses were quite species-specific in pathogenicity for animals and tissue cultures.