The dynamics of insulin release from mouse pancreatic islet cells in suspension

The overall dynamics of glucose-induced insulin release was strikingly similar in dispersed cells and intact islets perifused in parallel. Both preparations exhibited a latency of 1-2 min, after which period there was a brisk rise of insulin release followed by a sustained second phase. During the s...

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Bibliographic Details
Published in:Pflügers Archiv 1976-01, Vol.366 (2-3), p.185-188
Main Authors: Idahl, L A, Lernmark, A, Sehlin, J, Täljedal, I B
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Epinephrine - pharmacology
Female
Glucose - metabolism
Glucose - pharmacology
In Vitro Techniques
Insulin - metabolism
Insulin Secretion
Islets of Langerhans - metabolism
Kinetics
Mice
Mice, Obese
Microbial Collagenase
Oxidation-Reduction
Perfusion
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Summary:The overall dynamics of glucose-induced insulin release was strikingly similar in dispersed cells and intact islets perifused in parallel. Both preparations exhibited a latency of 1-2 min, after which period there was a brisk rise of insulin release followed by a sustained second phase. During the second phase, insulin release from dispersed cells attained a stable plateau rate, whereas the release from intact islets continued to rise. Epinephrine (1 muM) inhibited the release in both preparations, but the return to basal rate was faster in the dispersed cells than in the intact islets. The dispersed cells oxidized glucose at a constant rate for at least 60 min; the glucose oxidation was markedly sensitive to changes of the glucose concentration in the range of 3-20 mM.
ISSN:0031-6768
1432-2013
DOI:10.1007/BF00585876