Long-Term Propagation of a Murine Leukemia Virus in an Established Cell Line

Cells derived from the spleens of suckling strain BALB/cAnN mice were established in long-term culture. The cells retained their susceptibility to infection by the Moloney leukemia virus. Cultures exposed to cell-free preparations of virus after 7.5 and 14 months of cultivation did not appear to be...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1964-08, Vol.33 (2), p.363-371
Main Authors: Manaker, Robert A., Jensen, Erling M., Korol, Wolodymyr
Format: Article
Language:English
Subjects:
Animals
Cell Line
Leukemia
Leukemia Virus, Murine
Leukemia, Experimental
Mice
Old Medline
Oncogenic Viruses
Research Design
Spleen
Tissue Culture Techniques
Virus Cultivation
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Summary:Cells derived from the spleens of suckling strain BALB/cAnN mice were established in long-term culture. The cells retained their susceptibility to infection by the Moloney leukemia virus. Cultures exposed to cell-free preparations of virus after 7.5 and 14 months of cultivation did not appear to be as sensitive to infection as were primary spleen cell cultures, since a comparatively longer period of incubation after exposure to virus was required before samples of culture fluids were leukemogenic for mice. The infected cells (MT-77) continuously released virus during 4 years of observation. No overt cytopathic changes were noted. The MT-77 line of cells, which could be propagated in suspension in 8-liter fermentors, provided large quantities of virus-containing fluids. When 8 × 105 cells were inoculated subcutaneously into baby mice, tumors developed at the site of implantation. No conclusions may be drawn concerning the role of the virus in the neoplastic change.
ISSN:0027-8874
1460-2105
1460-2105
DOI:10.1093/jnci/33.2.363