X-ray diffraction studies of circular superhelical DNA at 300-10,000- resolution

X-ray diffraction studies on circular superhelical DNA from bacteriophage PM2 at a very low scattering angle show that it is possible to measure the superhelical scattering function of the molecule. The results suggest that in addition to the primary supercoil, a higher order of supercoiling of the...

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Bibliographic Details
Published in:Nature (London) 1976-11, Vol.264 (5583), p.231-234
Main Authors: Brady, G. W, Fein, D. B, Brumberger, H
Format: Article
Language:English
Subjects:
Coliphages
DNA, Circular
DNA, Viral
Humanities and Social Sciences
Models, Structural
multidisciplinary
Nucleic Acid Conformation
Science
Science (multidisciplinary)
X-Ray Diffraction
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Summary:X-ray diffraction studies on circular superhelical DNA from bacteriophage PM2 at a very low scattering angle show that it is possible to measure the superhelical scattering function of the molecule. The results suggest that in addition to the primary supercoil, a higher order of supercoiling of the DNA is present, an effect which can be interpreted with a simple analogue.
ISSN:0028-0836
1476-4687
DOI:10.1038/264231a0