Di- and trimethylated congeners of 7-methylguanine in Sindbis virus mRNA

IN the past two years it has become clear that most animal messenger RNA—both cellular 1–6 and viral 7–11 —is “capped” by 7-methylguanosine (m 7 G) residues present in inverted nucleotide linkage at 5′ termini of the general form m 7 G (5′)pppNm. At least for certain mRNAs the capping m 7 G has an i...

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Bibliographic Details
Published in:Nature (London) 1976-11, Vol.264 (5582), p.190-191
Main Authors: HSUCHEN, CHUEN-CHIN, DUBIN, DONALD T
Format: Article
Language:English
Subjects:
Guanine Nucleotides - analysis
Humanities and Social Sciences
letter
Methylation
multidisciplinary
RNA, Messenger - analysis
RNA, Viral - analysis
Science
Science (multidisciplinary)
Sindbis Virus - analysis
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Summary:IN the past two years it has become clear that most animal messenger RNA—both cellular 1–6 and viral 7–11 —is “capped” by 7-methylguanosine (m 7 G) residues present in inverted nucleotide linkage at 5′ termini of the general form m 7 G (5′)pppNm. At least for certain mRNAs the capping m 7 G has an important role in mRNA-ribosome binding and thus in initiation of protein synthesis 12–15 . We now report novel forms of capped 5′ termini on the main mRNA, ‘26S’ RNA, coded for by the animal alphavirus, Sindbis. These termini contain, in addition to the usual m 7 G, its hypermodified congeners m 2 2,7 G and m 3 2,2,7 G. This last compound has been previously described only in small nuclear (sn) RNAs of unknown function 16,17 , whereas m 2 2,7 G has not been described previously.
ISSN:0028-0836
1476-4687
DOI:10.1038/264190a0