SPECTROPHOTOMETRIC ANALYZERS FOR DISC ELECTROPHORESIS: STUDIES OF YEAST CYTOCHROME c

Summary Attachments to the Beckman DU and DK‐2 spectrophotometers are described for use in recording the disc electrophoresis patterns of colored materials at various wavelengths of light. The attachment to the DK‐2 instrument allows automatic recording of the pattern and can in addition be used to...

Full description

Saved in:
Bibliographic Details
Published in:Annals of the New York Academy of Sciences 1964-12, Vol.121 (2), p.600-615
Main Authors: Taber, Harry W., Sherman, Fred
Format: Article
Language:English
Subjects:
Chromatography
Cytochromes
Cytochromes c
Electrophoresis
Electrophoresis, Disc
Old Medline
Saccharomyces
Spectrophotometry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Attachments to the Beckman DU and DK‐2 spectrophotometers are described for use in recording the disc electrophoresis patterns of colored materials at various wavelengths of light. The attachment to the DK‐2 instrument allows automatic recording of the pattern and can in addition be used to obtain a full wavelength scan of a given electrophoretic component. A study of the electrophoresis of yeast cytochrome c with these instruments showed that this hemoprotein can be readily converted to derivative forms by treatment with hydroquinone and by washing the resin‐bound enzyme with dilute buffer.
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1964.tb14229.x