SPECTROPHOTOMETRIC ANALYZERS FOR DISC ELECTROPHORESIS: STUDIES OF YEAST CYTOCHROME c

Summary Attachments to the Beckman DU and DK‐2 spectrophotometers are described for use in recording the disc electrophoresis patterns of colored materials at various wavelengths of light. The attachment to the DK‐2 instrument allows automatic recording of the pattern and can in addition be used to...

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Published in:Annals of the New York Academy of Sciences 1964-12, Vol.121 (2), p.600-615
Main Authors: Taber, Harry W., Sherman, Fred
Format: Article
Language:English
Subjects:
Chromatography
Cytochromes
Cytochromes c
Electrophoresis
Electrophoresis, Disc
Old Medline
Saccharomyces
Spectrophotometry
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container_title Annals of the New York Academy of Sciences
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creator Taber, Harry W.
Sherman, Fred
description Summary Attachments to the Beckman DU and DK‐2 spectrophotometers are described for use in recording the disc electrophoresis patterns of colored materials at various wavelengths of light. The attachment to the DK‐2 instrument allows automatic recording of the pattern and can in addition be used to obtain a full wavelength scan of a given electrophoretic component. A study of the electrophoresis of yeast cytochrome c with these instruments showed that this hemoprotein can be readily converted to derivative forms by treatment with hydroquinone and by washing the resin‐bound enzyme with dilute buffer.
doi_str_mv 10.1111/j.1749-6632.1964.tb14229.x
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The attachment to the DK‐2 instrument allows automatic recording of the pattern and can in addition be used to obtain a full wavelength scan of a given electrophoretic component. A study of the electrophoresis of yeast cytochrome c with these instruments showed that this hemoprotein can be readily converted to derivative forms by treatment with hydroquinone and by washing the resin‐bound enzyme with dilute buffer.</description><subject>Chromatography</subject><subject>Cytochromes</subject><subject>Cytochromes c</subject><subject>Electrophoresis</subject><subject>Electrophoresis, Disc</subject><subject>Old Medline</subject><subject>Saccharomyces</subject><subject>Spectrophotometry</subject><issn>0077-8923</issn><issn>1749-6632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1964</creationdate><recordtype>article</recordtype><recordid>eNqVkEFv2yAYQFG1qs26_YUJ7bCbXbAxmF4my3EaS24cGaoqvSCCiZQsWVKTaOm_L5HT7tzvwoH3PcQD4CdGIfZzuwoxIzygNI5CzCkJ93NMooiHxwsw-Lj6AgYIMRakPIqvwVfnVgjhKCXsClx7nKAkSQZAimmRy6aejmtZPxSyKXOYTbJq9lw0Ao7qBg5LkcOieqeaQpTiDgr5OCwLAesRnBWZkDCfyTofN94BzTdwudBrZ7-fzxvwOCpkPg6q-r7MsyowBHEUcKZN3Gqa4DnGyKA2MoRobVFCmP-BZq1tI2w4Z4m2hFNsNV4YhjmhxDCTxjfgV-_ddduXg3V7tVk6Y9dr_dduD06lMU39UA_e9aDpts51dqF23XKju1eFkTo1VSt1CqdO4dSpqTo3VUe__OP8ymG-se3_1XNED_zugX_LtX39hFpNZpmgCHlD0BuWbm-PHwbd_VGUxSxRT5N7VU1xJOVoqnj8BrQwjuc</recordid><startdate>196412</startdate><enddate>196412</enddate><creator>Taber, Harry W.</creator><creator>Sherman, Fred</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196412</creationdate><title>SPECTROPHOTOMETRIC ANALYZERS FOR DISC ELECTROPHORESIS: STUDIES OF YEAST CYTOCHROME c</title><author>Taber, Harry W. ; Sherman, Fred</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4090-97ac3da651b110c0d2c44aae0547663a7ded21c9975ae4961ea1fc719464c7c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1964</creationdate><topic>Chromatography</topic><topic>Cytochromes</topic><topic>Cytochromes c</topic><topic>Electrophoresis</topic><topic>Electrophoresis, Disc</topic><topic>Old Medline</topic><topic>Saccharomyces</topic><topic>Spectrophotometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taber, Harry W.</creatorcontrib><creatorcontrib>Sherman, Fred</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of the New York Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taber, Harry W.</au><au>Sherman, Fred</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SPECTROPHOTOMETRIC ANALYZERS FOR DISC ELECTROPHORESIS: STUDIES OF YEAST CYTOCHROME c</atitle><jtitle>Annals of the New York Academy of Sciences</jtitle><addtitle>Ann N Y Acad Sci</addtitle><date>1964-12</date><risdate>1964</risdate><volume>121</volume><issue>2</issue><spage>600</spage><epage>615</epage><pages>600-615</pages><issn>0077-8923</issn><eissn>1749-6632</eissn><abstract>Summary Attachments to the Beckman DU and DK‐2 spectrophotometers are described for use in recording the disc electrophoresis patterns of colored materials at various wavelengths of light. The attachment to the DK‐2 instrument allows automatic recording of the pattern and can in addition be used to obtain a full wavelength scan of a given electrophoretic component. A study of the electrophoresis of yeast cytochrome c with these instruments showed that this hemoprotein can be readily converted to derivative forms by treatment with hydroquinone and by washing the resin‐bound enzyme with dilute buffer.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>14240555</pmid><doi>10.1111/j.1749-6632.1964.tb14229.x</doi><tpages>16</tpages></addata></record>
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source Wiley Online (Archive)
subjects Chromatography
Cytochromes
Cytochromes c
Electrophoresis
Electrophoresis, Disc
Old Medline
Saccharomyces
Spectrophotometry
title SPECTROPHOTOMETRIC ANALYZERS FOR DISC ELECTROPHORESIS: STUDIES OF YEAST CYTOCHROME c
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