Surface membrane nucleoside triphosphatase activity and tumorigenicity of cultured liver epithelial cells

A cell surface-located nucleoside triphosphatase activity can be assayed in liver epithelial cultures in situ with the incubation of intact cells in a medium containing [gamma-32P]adenosine triphosphate and correlated with the tumorigenicity of these cells in neonatal Wistar rats. The ectoenzyme act...

Full description

Saved in:
Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1976-12, Vol.36 (12), p.4491-4499
Main Authors: Karasaki, S, Okigaki, T
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - metabolism
Animals
Calcium - metabolism
Cell Division
Cell Line
Cell Membrane - enzymology
Cell Membrane - ultrastructure
Cell Transformation, Neoplastic
Cells, Cultured
Epithelial Cells
Epithelium - enzymology
Epithelium - ultrastructure
Histocytochemistry
Liver - enzymology
Liver - ultrastructure
Magnesium - metabolism
Neoplasms, Experimental - etiology
Rats
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A cell surface-located nucleoside triphosphatase activity can be assayed in liver epithelial cultures in situ with the incubation of intact cells in a medium containing [gamma-32P]adenosine triphosphate and correlated with the tumorigenicity of these cells in neonatal Wistar rats. The ectoenzyme activity of normal diploid cell lines is minimal, whereas a considerably high activity has been found in all tumorigenic cell lines tested. The optimum condition for the adenosinetriphosphatase activity is physiological with regard to osmolarity, ionic composition, pH, and substrate concentration in the medium. The enzyme is significantly stimulated by Ca2+, and its activation is controlled by Mg2+. Histochemical examinations indicate that glutaraldehyde-fixed cells of tumorigenic lines have Ca2+-stimulated adenosinetriphosphatase activity on the external surface. The isotopic assay of adenosine triphosphate hydrolysis by intact cells may provide a rapid method for screening oncogenesis in vitro of liver epithelial cells.
ISSN:0008-5472