Detection, Quantitation, and Characterization of the Major Internal Virion Antigen of the Bovine Leukemia Virus by Radioimmunoassay

The major internal polypeptide of the bovine leukemia virus (BLV) was purified to homogeneity with the use of gel filtration and affinity chromatography. Like previous results, the protein had a molecular weight of 25,000 daltons as determined by electrophoresis in polyacrylamide gels with sodium do...

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Published in:JNCI : Journal of the National Cancer Institute 1976-10, Vol.57 (4), p.875-882
Main Authors: McDonald, Hugh C., Ferrer, Jorge F.
Format: Article
Language:English
Subjects:
Animals
Antigens, Viral - analysis
Binding, Competitive
Cattle
Cell Line
Epitopes
Leukemia Virus, Bovine - immunology
Molecular Weight
Radioimmunoassay
Retroviridae - immunology
Viral Proteins - analysis
Viral Proteins - immunology
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Ferrer, Jorge F.
description The major internal polypeptide of the bovine leukemia virus (BLV) was purified to homogeneity with the use of gel filtration and affinity chromatography. Like previous results, the protein had a molecular weight of 25,000 daltons as determined by electrophoresis in polyacrylamide gels with sodium dodecyl sulfate, More than 90% of the 125I-labeled protein was precipitated by bovine sera that reacted in immunofluorescence tests with acetone-fixed BLV-infected cells. In contrast, minimal precipitation (
doi_str_mv 10.1093/jnci/57.4.875
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Competitive radioimmunoassay(s) (RIA) also failed to detect cross-reactions between BLV p25 protein and the internal polypeptides of other mammalian and' avian oncornaviruses, M-PMV, or foamy-like BSV. 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Competitive radioimmunoassay(s) (RIA) also failed to detect cross-reactions between BLV p25 protein and the internal polypeptides of other mammalian and' avian oncornaviruses, M-PMV, or foamy-like BSV. 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source Oxford University Press:Jisc Collections:Oxford Journal Archive: Access period 2024-2025
subjects Animals
Antigens, Viral - analysis
Binding, Competitive
Cattle
Cell Line
Epitopes
Leukemia Virus, Bovine - immunology
Molecular Weight
Radioimmunoassay
Retroviridae - immunology
Viral Proteins - analysis
Viral Proteins - immunology
title Detection, Quantitation, and Characterization of the Major Internal Virion Antigen of the Bovine Leukemia Virus by Radioimmunoassay
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