Sedimentation studies on membrane vesicles

Analytical centrifugation was used in order to investigate the size distribution of vesicles in various membrane preparations. Under certain conditions depending among others on the speed of rotation and the temperature, a sedimentation profile was observed that was characteristic for membraneous ma...

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Bibliographic Details
Published in:Molecular biology reports 1976-09, Vol.3 (1), p.81-86
Main Authors: Boom, J, Bont, W S, Hofs, H P, De Vries, M
Format: Article
Language:English
Subjects:
Cell Fractionation
Cell Membrane - ultrastructure
Centrifugation
Endoplasmic Reticulum - ultrastructure
Membranes - ultrastructure
Nuclear Envelope - ultrastructure
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Summary:Analytical centrifugation was used in order to investigate the size distribution of vesicles in various membrane preparations. Under certain conditions depending among others on the speed of rotation and the temperature, a sedimentation profile was observed that was characteristic for membraneous material. Since the membrane vesicles exhibited a discontinuous distribution of sedimentation coefficients it was concluded that membrane vesicles only occur in a few size classes.
ISSN:0301-4851
1573-4978
DOI:10.1007/BF00357212