AN UNUSUAL ISOZYME OF LACTATE DEHYDROGENASE IN MATURE TESTES: LOCALIZATION, ONTOGENY, AND KINETIC PROPERTIES

Summary One or more electrophoretically unusual isozymes of LDH have been demonstrated in homogenates of mature testes from some species. Analyses of the isozymic composition of many other tissues, developing testes, and seminal fluid revealed that these unusual forms, or “band X” isozymes, are pres...

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Bibliographic Details
Published in:Annals of the New York Academy of Sciences 1964-12, Vol.121 (2), p.571-588
Main Authors: Zinkham, William H., Blanco, Antonio, Clowry Jr, Lawrence J.
Format: Article
Language:English
Subjects:
Animals
Electrophoresis
Guinea Pigs
Histocytochemistry
Humans
Isoenzymes
Kinetics
L-Lactate Dehydrogenase
Male
Old Medline
Physiological Phenomena
Physiology, Comparative
Rabbits
Spermatozoa
Testis
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Summary:Summary One or more electrophoretically unusual isozymes of LDH have been demonstrated in homogenates of mature testes from some species. Analyses of the isozymic composition of many other tissues, developing testes, and seminal fluid revealed that these unusual forms, or “band X” isozymes, are present only in postpubertal testes and are localized in the differentiating germ cells of the seminiferous tubules. Rates of reactivity with certain alpha‐hydroxy acids and analogs of nicotinamide adenine dinucleotide differentiated some of the “band X” isozymes from the usual LDH isozymes. Results of dissociation and recombination studies on homogenates of human and rabbit testes indicated a similarity in structure between “band X” and the other isozymes. The ontogeny and characteristic distribution of the “band X” isozymes suggested that each fulfills a specific metabolic function. As yet the nature of this metabolic assignment has not been determined.
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1964.tb14227.x