Persistent Infection with Bovine Herpesvirus-1 (Infectious Bovine Rhinotracheitis Virus) in Cultured Hamster Cells

Bovine herpesvirus-1 infection in hamster embryo cells was found to be dependent upon input multiplicity; productive infection was achieved at input multiplicities greater than one, while persistent infection was established when input multiplicities were about 0.5. This persistence was characterize...

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Bibliographic Details
Published in:In Vitro 1976-10, Vol.12 (10), p.682-686
Main Authors: Michalski, F. J., Hsiung, G. D.
Format: Article
Language:English
Subjects:
Animals
Cell culture techniques
Cell growth
Cell lines
Cricetinae
Culture Techniques
Cultured cells
Cytopathogenic Effect, Viral
Embryonic cells
Fibroblasts
Herpesviridae
Herpesvirus 1, Bovine - growth & development
Herpesvirus 1, Bovine - isolation & purification
Infections
L cells
Mesocricetus
Ungulates
Virus Replication
Viruses
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Summary:Bovine herpesvirus-1 infection in hamster embryo cells was found to be dependent upon input multiplicity; productive infection was achieved at input multiplicities greater than one, while persistent infection was established when input multiplicities were about 0.5. This persistence was characterized by a noncyclic, minimal degree of cytopathic effect with a low level of released virus. Maintenance of the persistently infected cultures did not require external supportive measures. Subcultivation of the persistently infected cultures led to virus replication followed by CPE and then cell regrowth. Within 3 to 4 weeks after subcultivation a persistent infection was re-established. The possible mechanism for the bovine herpesvirus persistence in hamster cells is discussed.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02797471