Amino acid sequence of phospholipase A2 from horse pancreas

The complete amino acid sequence of phosphlipase A2 (EC 3.1.1.4) from horse pancreas was determined. The protein controls of a single polypeptide chain of 125 amino acids and has a molecular weight of 13,927. The chain is crosslinked by seven disulfide bridges. The sequence was determined by automat...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1977-02, Vol.252 (4), p.1189-1196
Main Authors: Evenberg, A, Meyer, H, Gaastra, W, Verhelj, H.M, Haas, G.H. de
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acids - analysis
animal science
Animals
Horses
livestock
Molecular Weight
Oxidation-Reduction
Pancreas - enzymology
Peptide Fragments - analysis
Peptide Hydrolases
Phospholipases
zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The complete amino acid sequence of phosphlipase A2 (EC 3.1.1.4) from horse pancreas was determined. The protein controls of a single polypeptide chain of 125 amino acids and has a molecular weight of 13,927. The chain is crosslinked by seven disulfide bridges. The sequence was determined by automated Edman degradation of the intact protein and several of the large peptide fragments. Smaller peptides were analyzed by manual Edman degradation. Fragmentation of the peptide chain was accomplished by enzymatic digestion with trypsin, chymotrypsin, and thermolysin. The final overlap was found by digestion of the polypeptide with a staphylococcal protease specific for glutamoyl bonds. Phospholipase A2 from horse pancreas shows homology to snake venom phospholipases A2 and to the enzyme from porcine pancreas, provided that the published amino acid sequence of the porcine phospholipase A2 is revised to some extent.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)40639-9