Bone Cells: A Serum-Free Medium Supports Proliferation in Primary Culture

Bone cells isolated from embryonic rat calvaria increase in number two-to threefold when cultured at high, but not at low, population densities in a serumfree medium that contains albumin. Cultured cells respond to parathyroid hormone and exhibit a marked rise in alkaline phosphatase activity during...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1978-02, Vol.199 (4328), p.542-544
Main Authors: Burks, James K., Peck, William A.
Format: Article
Language:English
Subjects:
Amino acids
Animals
Bone and Bones - cytology
Bone Development
Bones
Cell aggregates
Cell culture techniques
Cell Differentiation
Cell Division
Cell growth
Cell lines
Cell separation
Cells, Cultured
Culture Media
Cultured cells
Hormones
Phosphatases
Rats
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Summary:Bone cells isolated from embryonic rat calvaria increase in number two-to threefold when cultured at high, but not at low, population densities in a serumfree medium that contains albumin. Cultured cells respond to parathyroid hormone and exhibit a marked rise in alkaline phosphatase activity during proliferation, which suggests the progressive differentiation or preferential growth of osteoblast-like cells.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.564080