Loading…

Specific binding sites for oestrogen at the outer surfaces of isolated endometrial cells

OESTROGENS are more readily accumulated and retained in responsive cells than in cells that are not their targets 1 . Cytoplasmic macromolecules 2 which specifically interact with oestradiol and other steroid hormones seem to mediate transfer of the agonist to the nuclear chromatin, where the comple...

Full description

Saved in:
Bibliographic Details
Published in:Nature (London) 1977-01, Vol.265 (5589), p.69-72
Main Authors: PIETRAS, RICHARD J, SZEGO, CLARA M
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c334t-2d49069664dd2297a36e2edc52d0ca7eb0c1c233d7932a94b486c4babcc83e3f3
cites cdi_FETCH-LOGICAL-c334t-2d49069664dd2297a36e2edc52d0ca7eb0c1c233d7932a94b486c4babcc83e3f3
container_end_page 72
container_issue 5589
container_start_page 69
container_title Nature (London)
container_volume 265
creator PIETRAS, RICHARD J
SZEGO, CLARA M
description OESTROGENS are more readily accumulated and retained in responsive cells than in cells that are not their targets 1 . Cytoplasmic macromolecules 2 which specifically interact with oestradiol and other steroid hormones seem to mediate transfer of the agonist to the nuclear chromatin, where the complex is believed to promote expression of the phenotypic effects 3–6 . It is generally assumed that the hormone diffuses passively to “cytoplasmic” receptors which determine the cellular specificity of response 7 . But some experiments indicate that steroid hormones interact with components of biological membranes and may enter their respective target cells by a membrane-mediated process 8–12 which is saturable and temperature-dependent 13–17 . We have investigated steroid-binding components associated with the plasma membranes of cells isolated from endometrium, liver and intestinal mucosa. Endometrial and liver cells show substantial binding to oestrogen immobilised by covalent linkage to an inert support, while intestinal cells have no such binding sites. The relative quantity of these steroid receptors at the outer surfaces of cells from diverse tissues corresponds well with the capacity of a given cell to accumulate and retain oestrogen.
doi_str_mv 10.1038/265069a0
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_83754293</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>83754293</sourcerecordid><originalsourceid>FETCH-LOGICAL-c334t-2d49069664dd2297a36e2edc52d0ca7eb0c1c233d7932a94b486c4babcc83e3f3</originalsourceid><addsrcrecordid>eNptkDtPwzAUhS3EqxQkfgBCnhAMAcd2nGREFS-pEgMgsUWOfVNcpXaxnYF_j6uUTkx3OJ8-nXsQOs_JbU5YdUdFQUQtyR6a5LwUGRdVuY8mhNAqIxUTx-gkhCUhpMhLfoQOK8Yp5xP0-bYGZTqjcGusNnaBg4kQcOc8dhCidwuwWEYcvwC7IYLHYfCdVIlxHTbB9TKCxmC1W0H0RvZYQd-HU3TQyT7A2fZO0cfjw_vsOZu_Pr3M7ueZYozHjGpep-JCcK0prUvJBFDQqqCaKFlCS1SuKGO6rBmVNW95JRRvZatUxYB1bIquRu_au-8hNW5WJmwaSAtuCE3FyoLTmiXwegSVdyF46Jq1Nyvpf5qcNJsNm78NE3qxdQ7tCvQOHEdL8c0YhxTYBfhm6QZv05f_qS5H1so4eNipdsAvCsyDxQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>83754293</pqid></control><display><type>article</type><title>Specific binding sites for oestrogen at the outer surfaces of isolated endometrial cells</title><source>Nature</source><creator>PIETRAS, RICHARD J ; SZEGO, CLARA M</creator><creatorcontrib>PIETRAS, RICHARD J ; SZEGO, CLARA M</creatorcontrib><description>OESTROGENS are more readily accumulated and retained in responsive cells than in cells that are not their targets 1 . Cytoplasmic macromolecules 2 which specifically interact with oestradiol and other steroid hormones seem to mediate transfer of the agonist to the nuclear chromatin, where the complex is believed to promote expression of the phenotypic effects 3–6 . It is generally assumed that the hormone diffuses passively to “cytoplasmic” receptors which determine the cellular specificity of response 7 . But some experiments indicate that steroid hormones interact with components of biological membranes and may enter their respective target cells by a membrane-mediated process 8–12 which is saturable and temperature-dependent 13–17 . We have investigated steroid-binding components associated with the plasma membranes of cells isolated from endometrium, liver and intestinal mucosa. Endometrial and liver cells show substantial binding to oestrogen immobilised by covalent linkage to an inert support, while intestinal cells have no such binding sites. The relative quantity of these steroid receptors at the outer surfaces of cells from diverse tissues corresponds well with the capacity of a given cell to accumulate and retain oestrogen.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/265069a0</identifier><identifier>PMID: 834244</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Binding Sites ; Cell Membrane - metabolism ; Endometrium - cytology ; Endometrium - metabolism ; Estradiol - metabolism ; Female ; Humanities and Social Sciences ; Intestinal Mucosa - cytology ; Intestinal Mucosa - metabolism ; letter ; Liver - cytology ; Liver - metabolism ; multidisciplinary ; Rats ; Receptors, Estrogen - metabolism ; Science ; Science (multidisciplinary)</subject><ispartof>Nature (London), 1977-01, Vol.265 (5589), p.69-72</ispartof><rights>Springer Nature Limited 1977</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-2d49069664dd2297a36e2edc52d0ca7eb0c1c233d7932a94b486c4babcc83e3f3</citedby><cites>FETCH-LOGICAL-c334t-2d49069664dd2297a36e2edc52d0ca7eb0c1c233d7932a94b486c4babcc83e3f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/834244$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PIETRAS, RICHARD J</creatorcontrib><creatorcontrib>SZEGO, CLARA M</creatorcontrib><title>Specific binding sites for oestrogen at the outer surfaces of isolated endometrial cells</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>OESTROGENS are more readily accumulated and retained in responsive cells than in cells that are not their targets 1 . Cytoplasmic macromolecules 2 which specifically interact with oestradiol and other steroid hormones seem to mediate transfer of the agonist to the nuclear chromatin, where the complex is believed to promote expression of the phenotypic effects 3–6 . It is generally assumed that the hormone diffuses passively to “cytoplasmic” receptors which determine the cellular specificity of response 7 . But some experiments indicate that steroid hormones interact with components of biological membranes and may enter their respective target cells by a membrane-mediated process 8–12 which is saturable and temperature-dependent 13–17 . We have investigated steroid-binding components associated with the plasma membranes of cells isolated from endometrium, liver and intestinal mucosa. Endometrial and liver cells show substantial binding to oestrogen immobilised by covalent linkage to an inert support, while intestinal cells have no such binding sites. The relative quantity of these steroid receptors at the outer surfaces of cells from diverse tissues corresponds well with the capacity of a given cell to accumulate and retain oestrogen.</description><subject>Animals</subject><subject>Binding Sites</subject><subject>Cell Membrane - metabolism</subject><subject>Endometrium - cytology</subject><subject>Endometrium - metabolism</subject><subject>Estradiol - metabolism</subject><subject>Female</subject><subject>Humanities and Social Sciences</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>letter</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>multidisciplinary</subject><subject>Rats</subject><subject>Receptors, Estrogen - metabolism</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><recordid>eNptkDtPwzAUhS3EqxQkfgBCnhAMAcd2nGREFS-pEgMgsUWOfVNcpXaxnYF_j6uUTkx3OJ8-nXsQOs_JbU5YdUdFQUQtyR6a5LwUGRdVuY8mhNAqIxUTx-gkhCUhpMhLfoQOK8Yp5xP0-bYGZTqjcGusNnaBg4kQcOc8dhCidwuwWEYcvwC7IYLHYfCdVIlxHTbB9TKCxmC1W0H0RvZYQd-HU3TQyT7A2fZO0cfjw_vsOZu_Pr3M7ueZYozHjGpep-JCcK0prUvJBFDQqqCaKFlCS1SuKGO6rBmVNW95JRRvZatUxYB1bIquRu_au-8hNW5WJmwaSAtuCE3FyoLTmiXwegSVdyF46Jq1Nyvpf5qcNJsNm78NE3qxdQ7tCvQOHEdL8c0YhxTYBfhm6QZv05f_qS5H1so4eNipdsAvCsyDxQ</recordid><startdate>19770106</startdate><enddate>19770106</enddate><creator>PIETRAS, RICHARD J</creator><creator>SZEGO, CLARA M</creator><general>Nature Publishing Group UK</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19770106</creationdate><title>Specific binding sites for oestrogen at the outer surfaces of isolated endometrial cells</title><author>PIETRAS, RICHARD J ; SZEGO, CLARA M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c334t-2d49069664dd2297a36e2edc52d0ca7eb0c1c233d7932a94b486c4babcc83e3f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Animals</topic><topic>Binding Sites</topic><topic>Cell Membrane - metabolism</topic><topic>Endometrium - cytology</topic><topic>Endometrium - metabolism</topic><topic>Estradiol - metabolism</topic><topic>Female</topic><topic>Humanities and Social Sciences</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>letter</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>multidisciplinary</topic><topic>Rats</topic><topic>Receptors, Estrogen - metabolism</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PIETRAS, RICHARD J</creatorcontrib><creatorcontrib>SZEGO, CLARA M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PIETRAS, RICHARD J</au><au>SZEGO, CLARA M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific binding sites for oestrogen at the outer surfaces of isolated endometrial cells</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1977-01-06</date><risdate>1977</risdate><volume>265</volume><issue>5589</issue><spage>69</spage><epage>72</epage><pages>69-72</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>OESTROGENS are more readily accumulated and retained in responsive cells than in cells that are not their targets 1 . Cytoplasmic macromolecules 2 which specifically interact with oestradiol and other steroid hormones seem to mediate transfer of the agonist to the nuclear chromatin, where the complex is believed to promote expression of the phenotypic effects 3–6 . It is generally assumed that the hormone diffuses passively to “cytoplasmic” receptors which determine the cellular specificity of response 7 . But some experiments indicate that steroid hormones interact with components of biological membranes and may enter their respective target cells by a membrane-mediated process 8–12 which is saturable and temperature-dependent 13–17 . We have investigated steroid-binding components associated with the plasma membranes of cells isolated from endometrium, liver and intestinal mucosa. Endometrial and liver cells show substantial binding to oestrogen immobilised by covalent linkage to an inert support, while intestinal cells have no such binding sites. The relative quantity of these steroid receptors at the outer surfaces of cells from diverse tissues corresponds well with the capacity of a given cell to accumulate and retain oestrogen.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>834244</pmid><doi>10.1038/265069a0</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0028-0836
ispartof Nature (London), 1977-01, Vol.265 (5589), p.69-72
issn 0028-0836
1476-4687
language eng
recordid cdi_proquest_miscellaneous_83754293
source Nature
subjects Animals
Binding Sites
Cell Membrane - metabolism
Endometrium - cytology
Endometrium - metabolism
Estradiol - metabolism
Female
Humanities and Social Sciences
Intestinal Mucosa - cytology
Intestinal Mucosa - metabolism
letter
Liver - cytology
Liver - metabolism
multidisciplinary
Rats
Receptors, Estrogen - metabolism
Science
Science (multidisciplinary)
title Specific binding sites for oestrogen at the outer surfaces of isolated endometrial cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T15%3A36%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Specific%20binding%20sites%20for%20oestrogen%20at%20the%20outer%20surfaces%20of%20isolated%20endometrial%20cells&rft.jtitle=Nature%20(London)&rft.au=PIETRAS,%20RICHARD%20J&rft.date=1977-01-06&rft.volume=265&rft.issue=5589&rft.spage=69&rft.epage=72&rft.pages=69-72&rft.issn=0028-0836&rft.eissn=1476-4687&rft_id=info:doi/10.1038/265069a0&rft_dat=%3Cproquest_cross%3E83754293%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c334t-2d49069664dd2297a36e2edc52d0ca7eb0c1c233d7932a94b486c4babcc83e3f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=83754293&rft_id=info:pmid/834244&rfr_iscdi=true