Chondroitin Sulfate: Inhibition of Synthesis by Puromycin

Puromycin reversibly inhibits synthesis of chondroitin sulfate by vertebral chondrocytes from 10-day-old chick embryos. Treatment of these cells with puromycin for 5 hours does not effect viability or capacity to multiply on subsequent release from their matrix. It is suggested that synthesis of thi...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1965-09, Vol.149 (3689), p.1263-1265
Main Authors: De La Haba, G., Holtzer, H.
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Carbon Isotopes
Cartilage - cytology
Cartilage - embryology
Cartilage - metabolism
Chemical suspensions
Chick Embryo - metabolism
Chromomycins
DNA
Embryos
Feedback
Glycosaminoglycans - biosynthesis
Homogenization
In Vitro Techniques
Liver
Microelectromechanical systems
Protein Biosynthesis
Protein synthesis
Puromycin - pharmacology
Sulfates
Sulfur Isotopes
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Description
Summary:Puromycin reversibly inhibits synthesis of chondroitin sulfate by vertebral chondrocytes from 10-day-old chick embryos. Treatment of these cells with puromycin for 5 hours does not effect viability or capacity to multiply on subsequent release from their matrix. It is suggested that synthesis of this polysaccharide is coupled with that of protein; there may be a feedback control in its synthesis.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.149.3689.1263