The RNA primer synthesized by primase to initiate phage G4 DNA replication

With phage G4 DNA as template, primase (the dnaG protein) synthesizes a 26- to 29-residue RNA transcript at the origin of replication. The sequence starts with ATP and contains a hairpin region of one A-U and seven G-C base pairs. Covalent linkage between the RNA and the newly synthesized complement...

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Bibliographic Details
Published in:The Journal of biological chemistry 1978-02, Vol.253 (3), p.765-769
Main Authors: Bouché, J P, Rowen, L, Kornberg, A
Format: Article
Language:English
Subjects:
Base Sequence
Coliphages - metabolism
DNA Replication
Escherichia coli - enzymology
RNA - biosynthesis
RNA Nucleotidyltransferases - metabolism
Templates, Genetic
Transcription, Genetic
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Summary:With phage G4 DNA as template, primase (the dnaG protein) synthesizes a 26- to 29-residue RNA transcript at the origin of replication. The sequence starts with ATP and contains a hairpin region of one A-U and seven G-C base pairs. Covalent linkage between the RNA and the newly synthesized complementary DNA chain indicates that the RNA transcript serves as a primer.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)38168-1