Acid Phosphatases

WE have been concerned with the isolation of acid phosphatases (orthophosphoric monoester phosphohydrolases, 3.1.3.2.) in quantities sufficient for chemical studies of these enzymes. The sources chosen were: the potato, the human prostate gland and human seminal plasma. We have found that the potato...

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Bibliographic Details
Published in:Nature (London) 1965-10, Vol.208 (5008), p.382-383
Main Authors: VERNON, C. A., GAULDIE, J., HANSON, JENNIFER M., HUMPHREYS, J. M., SMITH, P. E., LAWRENCE, A. J., BANKS, BARBARA E. C.
Format: Article
Language:English
Subjects:
Acid Phosphatase
Chemical Phenomena
Chemical Precipitation
Chemistry Techniques, Analytical
Chemistry, Physical
Chromatography
Dialysis
Humanities and Social Sciences
Humans
In Vitro Techniques
letter
Male
multidisciplinary
Prostate - enzymology
Science
Semen - enzymology
Vegetables - enzymology
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Summary:WE have been concerned with the isolation of acid phosphatases (orthophosphoric monoester phosphohydrolases, 3.1.3.2.) in quantities sufficient for chemical studies of these enzymes. The sources chosen were: the potato, the human prostate gland and human seminal plasma. We have found that the potato is a poor source and, although the enzyme has been obtained in a form which is homogeneous to starch-gel electrophoresis and to ultracentrifugation, the method is tedious and, because of the very large purification factor involved (about 25,000), leads to a low final yield (about 10 mg from 50 kg of potatoes). The best preparation previously reported was apparently associated with a purification factor of about 1,000 (ref. 1).
ISSN:0028-0836
1476-4687
DOI:10.1038/208382b0