Amino acid sequence of the L-2 light chain of rabbit skeletal muscle myosin

The L-2 light chain (DTNB light chain) was separated from rabbit skeletal muscle myosin and the amino acid sequence determined. In order to study the primary structure of the L-2 light chain, firstly, the tryptic peptides were separated from the L-2 light chain and the amino acid seqence determined....

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1977-01, Vol.81 (3), p.809-811
Main Authors: MATSUDA, Genji, MAITA, Tetsuo, SUZUYAMA, Yoji, SETOGUCHI, Masayuki, UMEGANE, Toshiyo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Chymotrypsin
Muscles - metabolism
Myosins
Peptide Fragments - analysis
Rabbits
Trypsin
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Summary:The L-2 light chain (DTNB light chain) was separated from rabbit skeletal muscle myosin and the amino acid sequence determined. In order to study the primary structure of the L-2 light chain, firstly, the tryptic peptides were separated from the L-2 light chain and the amino acid seqence determined. Then, to determine the arrangement of these tryptic peptides, enzymatic partial hydrolysis using 0.25 M acetic acid were carried out. The primary structure of the L-2 light chain thus obtained contains 168 amino acids.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a131520