One-to-one binding of a purified scorpion toxin to Na channels

• Published in: Nature (London) 1977-03, Vol.266 (5601), p.465-468
• Main Authors: OKAMOTO, HARUMASA, TAKAHASHI, KUNITARO, YAMASHITA, NAOHIDE
• Format: Article
• Language: English
• Subjects: Animals; Binding Sites; Calcium - metabolism; Cell Membrane - metabolism; Dose-Response Relationship, Drug; Electric Conductivity; Female; Humanities and Social Sciences; Kinetics; letter; Lithium - metabolism; Membrane Potentials - drug effects; multidisciplinary; Ovum - metabolism; Ovum - ultrastructure; Potassium - metabolism; Science; Scorpions; Sodium - metabolism; Toxins, Biological - isolation & purification; Toxins, Biological - metabolism; Toxins, Biological - pharmacology; Urochordata
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/266465a0

DETAILED elucidation of the molecular organisation which controls ionic flow through the excitable membranes has been prevented by a difficulty in isolating substances to which characteristic features of the ionic channels can be attributed 1–3 . In the study of the cholinergic receptor, polypeptide α-toxins from certain snakes have been successfully used for the identification and purification of nicotinic receptors 4 . Thus, agents analogous to snake toxins may be of a decisive value in the molecular approach to ionic channels, and toxins from certain scorpions are among the most promising candidates 5–8 . Scorpion toxins seem to act by modifying kinetic properties of Na channels as well as by suppressing the current through K channels 9–11 . But, no quantitative analysis was made on an electrophysiological basis to show the precise nature of the toxin-binding. We describe here a study of the effects of a toxin from Leiurus quinquestriatus on Na, Ca and K currents in the tunicate egg membrane, where each ionic current proved to be essentially identical with that in other excitable membranes 12,13 . The discrete critical membrane potentials for the activation of these currents in the egg facilitate discrimination of the respective currents only by adjusting potential steps in the voltage-clamp condition. Thus, quantitative aspects of the inactivation kinetics of Na current can be analysed conveniently in this preparation.