Fluorescence Polarization: Measurement with Ultraviolet-Polarizing Filters in a Spectrophotofluorometer

Ultraviolet-polarizing filters with large apertures facilitate the use of spectrophotofluorometers for measuring fluorescence polarization. Such filters (i) allow the use of polarized exciting light even in the ultraviolet region, (ii) make it possible to correct for grating anomalies which influenc...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1965-02, Vol.147 (3659), p.729-732
Main Authors: Chen, Raymond F., Bowman, Robert L.
Format: Article
Language:English
Subjects:
Absorption spectra
Coloring Agents
Dyes
Electrical resistivity
Equipment and Supplies
Filtration
Fluoresceins
Fluorescence
Fluorescence Polarization
Fluorometry
Light
Monochromators
Old Medline
Optical filters
Photometers
Polarized light
Spectrum Analysis
Transition temperature
Wavelengths
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Summary:Ultraviolet-polarizing filters with large apertures facilitate the use of spectrophotofluorometers for measuring fluorescence polarization. Such filters (i) allow the use of polarized exciting light even in the ultraviolet region, (ii) make it possible to correct for grating anomalies which influence the observed polarization, and (iii) because of good transmission properties allow the entrance of sufficient light so that small slits and dilute solutions can be used. Under optimum conditions, polarization can be measured with a precision of ±0.002. Results obtained are in excellent agreement with values cited elsewhere and indicate that standard spectrophotofluorometers can easily be adapted for accurate measurement of polarization.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.147.3659.729