Functional role of rice germin-like protein1 in regulation of plant height and disease resistance

The functional role of rice (Oryza sativa) germin-like protein1 (OsGLP1) was elucidated through development of transgenic plants involving endogenous gene silencing in rice and heterologous gene expression in tobacco. Usually, the single copy OsGLP1 gene in rice plant was found to be expressed predo...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2010-03, Vol.394 (1), p.178-183
Main Authors: Banerjee, Joydeep, Maiti, Mrinal K.
Format: Article
Language:English
Subjects:
Fungi
Germin-like protein
Glycoproteins - chemistry
Glycoproteins - genetics
Glycoproteins - physiology
Nicotiana - genetics
Oryza - genetics
Oryza - growth & development
Oryza - microbiology
Oryza sativa
OsGLP1
Plant Diseases - genetics
Plant Diseases - microbiology
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - physiology
Plants, Genetically Modified
Protein Structure, Tertiary
RNA, Small Interfering - genetics
Semi-dwarfing
siRNA
Superoxide dismutase
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Summary:The functional role of rice (Oryza sativa) germin-like protein1 (OsGLP1) was elucidated through development of transgenic plants involving endogenous gene silencing in rice and heterologous gene expression in tobacco. Usually, the single copy OsGLP1 gene in rice plant was found to be expressed predominantly in green vegetative tissues. The transgenic rice lines showed significant reduction in endogenous OsGLP1 expression due to 26nt siRNA-mediated gene silencing, displayed semi-dwarfism and were affected seriously by fungal diseases, compared to the untransformed plant. Structural homology modeling predicted a superoxide dismutase (SOD) domain in OsGLP1 protein which upon over-expression in transgenic tobacco plant clearly documented SOD activity. Our observations on the maintenance of cell dimension, cell wall-associated localization particularly in the sub-epidermal tissues and the SOD activity of OsGLP1 could explain its functional role in regulation of plant height and disease resistance in rice plant.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2010.02.142