Stable germline transformation of the malaria mosquito Anopheles stephensi

Anopheline mosquito species are obligatory vectors for human malaria, an infectious disease that affects hundreds of millions of people living in tropical and subtropical countries. The lack of a suitable gene transfer technology for these mosquitoes has hampered the molecular genetic analysis of th...

Full description

Saved in:
Bibliographic Details
Published in:Nature (London) 2000-06, Vol.405 (6789), p.959-962
Main Authors: Catteruccia, Flaminia, Nolan, Tony, Loukeris, Thanasis G., Blass, Claudia, Savakis, Charalambos, Kafatos, Fotis C., Crisanti, Andrea
Format: Article
Language:English
Subjects:
Animals
Anopheles - embryology
Anopheles - genetics
Anopheles stephensi
Aquatic insects
Bacteria
Biological and medical sciences
Biotechnology
Blotting, Southern
Culicidae
Deoxyribonucleic acid
DNA
DNA Transposable Elements
Female
Fundamental and applied biological sciences. Psychology
Genes, Insect
Genetic engineering
Genetic technics
Genetic Vectors
Genetics
Germ-Line Mutation
Humanities and Social Sciences
Humans
Infectious diseases
Insect Vectors - genetics
letter
Malaria
Malaria - parasitology
Male
Methods. Procedures. Technologies
Mosquitoes
multidisciplinary
Mutagenesis
Parasites
Physiology
Science
Science (multidisciplinary)
Transformation, Genetic
Transgenic animals
Transgenic animals and transgenic plants
Transposases - genetics
Transposases - metabolism
transposon Minos
Vector-borne diseases
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Anopheline mosquito species are obligatory vectors for human malaria, an infectious disease that affects hundreds of millions of people living in tropical and subtropical countries. The lack of a suitable gene transfer technology for these mosquitoes has hampered the molecular genetic analysis of their physiology, including the molecular interactions between the vector and the malaria parasite. Here we show that a transposon, based on the Minos element 1 and bearing exogenous DNA, can integrate efficiently and stably into the germ line of the human malaria vector Anopheles stephensi , through a transposase-mediated process.
ISSN:0028-0836
1476-4687
DOI:10.1038/35016096