RNA‐binding motif protein 24 regulates myogenin expression and promotes myogenic differentiation

The formation of muscle fibers involves sequential expression of many proteins that regulate key steps during myoblast‐to‐myotube transition. Myogenin is a major player in the initiation and maintenance of myogenic differentiation in a mouse myoblast cell line, C2C12. RNA‐binding proteins bind to sp...

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Bibliographic Details
Published in:Genes to cells : devoted to molecular & cellular mechanisms 2010-11, Vol.15 (11), p.1158-1167
Main Authors: Jin, Donghao, Hidaka, Kyoko, Shirai, Manabu, Morisaki, Takayuki
Format: Article
Language:English
Subjects:
3' Untranslated regions
Amino Acid Sequence
Animals
Cell Differentiation - genetics
Cell Line
Differentiation
Gene Expression
Gene Expression Regulation - physiology
Gene Knockdown Techniques
Half-Life
Mice
Muscle Development - physiology
Muscle Fibers, Skeletal - cytology
Muscles
Muscles - cytology
Myoblasts
Myoblasts - cytology
myogenesis
myogenin
Myogenin - genetics
Myogenin - metabolism
Plasmids
Post-transcription
Protein Processing, Post-Translational
RNA Interference
RNA, Messenger - metabolism
RNA-binding protein
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
RNA-mediated interference
Transcription, Genetic
Transfection
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Summary:The formation of muscle fibers involves sequential expression of many proteins that regulate key steps during myoblast‐to‐myotube transition. Myogenin is a major player in the initiation and maintenance of myogenic differentiation in a mouse myoblast cell line, C2C12. RNA‐binding proteins bind to specific target RNA sequences and regulate gene expression in a post‐transcriptional manner. This study demonstrates that RNA‐binding motif protein 24 (Rbm24) interacts with the 3′‐untranslated region of myogenin mRNA and affects its half‐life in C2C12 myogenesis. Knockdown of Rbm24 expression by RNA interference significantly decreased myogenin expression associated with the inhibition of myogenesis. In contrast, the overexpression of Rbm24 by stable transfection of a plasmid increased myogenin expression and had a positive effect on myogenic differentiation. Ectopic expression of myogenin was also able to restore myogenic differentiation in Rbm24‐knockdown cells. Together, our results suggest that Rbm24 binds to myogenin mRNA and regulates its stability in C2C12 cells. Rbm24 plays a crucial role in myogenic differentiation at least in part through a myogenin‐dependent post‐transcriptional regulatory pathway.
ISSN:1356-9597
1365-2443
DOI:10.1111/j.1365-2443.2010.01446.x