Isolation and analysis of amplified cDNA fragments during detection of unknown polymorphisms with temperature modulated heteroduplex chromatography

A procedure for isolation of polymerase chain reaction (PCR) fragments from an automated system for DNA fragment analysis is described. The technique is used for screening of unknown mutations or polymorphisms. Fragments are isolated during the analysis from the system by an automated unit for high-...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biotechnology 1999-06, Vol.11 (3), p.257-261
Main Authors: KUKLIN, A, MUNSON, K, TAYLOR, P, GJERDE, D
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Chromatography - methods
Deoxyribonucleic acid
Diverse techniques
DNA
DNA, Complementary - genetics
DNA, Complementary - isolation & purification
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Mutation
Polymerase Chain Reaction
Polymorphism
Polymorphism, Single Nucleotide
Temperature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A procedure for isolation of polymerase chain reaction (PCR) fragments from an automated system for DNA fragment analysis is described. The technique is used for screening of unknown mutations or polymorphisms. Fragments are isolated during the analysis from the system by an automated unit for high-throughput projects or manually when working with a few samples.
ISSN:1073-6085
1559-0305
DOI:10.1007/BF02788684