Dietary Supplementation with Fructooligosaccharides Attenuates Airway Inflammation Related to House Dust Mite Allergen in Mice

• Published in: International journal of immunopathology and pharmacology 2010-07, Vol.23 (3), p.727-735
• Main Authors: Yasuda, A., Inoue, K-I., Sanbongi, C., Yanagisawa, R., Ichinose, T., Yoshikawa, T., Takano, H.
• Format: Article
• Language: English
• Subjects: Administration, Inhalation; Allergens - administration & dosage; Allergens - toxicity; Animals; Antigens, Dermatophagoides - immunology; Bronchoalveolar Lavage Fluid - chemistry; Bronchoalveolar Lavage Fluid - cytology; Chemokines - biosynthesis; Cytokines - biosynthesis; Dermatophagoides farinae; Dermatophagoides pteronyssinus; Diet; Dietary Supplements; DNA - biosynthesis; DNA - genetics; Enzyme-Linked Immunosorbent Assay; Immunoglobulins - biosynthesis; Inflammation - drug therapy; Inflammation - pathology; Lung - metabolism; Lung - pathology; Male; Mice; Mice, Inbred C3H; Oligosaccharides - therapeutic use; Pyroglyphidae - immunology; Respiratory Hypersensitivity - drug therapy; Respiratory Hypersensitivity - pathology; Reverse Transcriptase Polymerase Chain Reaction
• ISSN: 0394-6320; 2058-7384
• DOI: 10.1177/039463201002300306

Fructooligosaccharides (FOS) are prebiotic supplements that can enhance immunological responses in the host to activate mucosal immunity, probably through regulation of gastrointestinal microflora. An area that has not been investigated, however, is the therapeutic potential of prebiotics on allergic airway diseases. The purpose of this study is to evaluate the effects of dietary supplementation with FOS on a murine model of airway inflammation induced by the house dust mite allergen Dermatophagoides farinae (Der f). Male C3H/HeN mice were intratracheally administered with Der f and were fed a diet containing 0% or 2.5% FOS ad libitum. Supplementation with FOS alleviated mite allergen-related airway inflammation characterized by eosinophilic inflammation and goblet cell hyperplasia, which was evidenced by cytological and histological examinations. In addition, the FOS-supplemented diet reduced the serum allergen-specific IgG1 level as compared with a control diet in the presence of the mite allergen. Moreover, FOS tended to suppress the expression of IL-5 and eotaxin in the lungs, which is enhanced by mite allergen. These results suggest that dietary supplementation with FOS can prevent/improve airway inflammation induced by the mite allergen. This effect can be at least partially associated with the inhibition of allergen-specific Ig production and probably with that of IL-5 and eotaxin expression.