Systematics of the subfamily Haplorchiinae (Trematoda: Heterphyidae), based on nuclear ribosomal DNA genes and ITS2 region

Abstract Phylogenetic relationships of 6 species in the trematode subfamily Haplorchiinae were analyzed using small and large subunit of ribosomal DNA genes (18S rDNA and 28S rDNA) and internal transcribed spacer subunit II (ITS2) region as molecular markers. Maximum Likelihood and Bayesian inferenc...

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Bibliographic Details
Published in:Parasitology international 2010-09, Vol.59 (3), p.460-465
Main Authors: Thaenkham, Urusa, Dekumyoy, Paron, Komalamisra, Chalit, Sato, Megumi, Dung, Do Trung, Waikagul, Jitra
Format: Article
Language:English
Subjects:
Animals
DNA, Helminth - analysis
DNA, Ribosomal Spacer - analysis
DNA, Ribosomal Spacer - genetics
Gastroenterology and Hepatology
Genes, rRNA
Haplorchis
Humans
Infectious Disease
Internal transcribed spacer subunit II
Nuclear ribosomal DNA genes
Phylogeny
Polymerase Chain Reaction
Procerovum
RNA, Ribosomal, 18S - genetics
RNA, Ribosomal, 28S - genetics
Sequence Analysis, DNA
Subfamily Haplorchiinae
Systematics
Trematoda
Trematoda - classification
Trematoda - genetics
Trematoda - isolation & purification
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Summary:Abstract Phylogenetic relationships of 6 species in the trematode subfamily Haplorchiinae were analyzed using small and large subunit of ribosomal DNA genes (18S rDNA and 28S rDNA) and internal transcribed spacer subunit II (ITS2) region as molecular markers. Maximum Likelihood and Bayesian inference analyses of combined rDNAs and ITS2 indicated a close relationship between the genera Haplorchis and Procerovum , while these two genera were distinct from Stellantchasmus falcatus . These phylogenetic relationships were consistent with the number of testes but not with the characters of the modification of the seminal vesicle or of the ventral sucker. Although three Haplorchis spp. were, together with Procerovum , in the same cluster, their mutual topology was incongruent between rDNA and ITS2 trees. Phylogenetic analyses using other molecular markers with more species are necessary to work out solid phylogenetic relationships among the species in this subfamily.
ISSN:1383-5769
1873-0329
DOI:10.1016/j.parint.2010.06.009