Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit

A simple and efficient method is described for isolating high quality RNA from bilberry fruit. The procedure is based on the use of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and beta-mercaptoethanol in an extraction buffer in order to eliminate the polysaccharides and p...

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Bibliographic Details
Published in:Molecular biotechnology 2001-10, Vol.19 (2), p.201-203
Main Authors: JAAKOLA, Laura, PIRTTILÄ, Anna Maria, HALONEN, Minna, HOHTOLA, Anja
Format: Article
Language:English
Subjects:
Ammonium
b-mercaptoethanol
Berries
Biological and medical sciences
Biotechnology
Cetrimonium Compounds - pharmacology
Diverse techniques
DNA, Complementary - metabolism
Fruits
Fundamental and applied biological sciences. Psychology
hexadecyltrimethyl ammonium bromide
Mercaptoethanol - pharmacology
Molecular and cellular biology
Molecular Biology - methods
Phenols
Pine trees
Plant tissues
Plasma Substitutes - pharmacology
polyvinylpyrrolidone
Povidone - pharmacology
Ribonucleic acid
RNA
RNA - analysis
RNA - isolation & purification
Saccharides
Vaccinium - metabolism
Vaccinium myrtillus
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Summary:A simple and efficient method is described for isolating high quality RNA from bilberry fruit. The procedure is based on the use of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and beta-mercaptoethanol in an extraction buffer in order to eliminate the polysaccharides and prevent the oxidation of phenolic compounds. This method is a modification of the one described for pine trees, and yields high-quality RNA suitable for cDNA based methodologies. This method is applicable for a variety of plant tissues.
ISSN:1073-6085
1559-0305
1073-6085
DOI:10.1385/mb:19:2:201