Alternaria alternata toxin detection by fluorescence derivatization and separation by high performance liquid chromatography

An improved high performance liquid chromatographic (HPLC) method was developed for the detection and quantitation of host-specific AA-toxin produced byAlternaria alternata, a water hyacinth pathogen. Precolumn derivatization of the toxin witho-phthalaldehyde (OPA) plus mercaptoethanol yielded highl...

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Bibliographic Details
Published in:Phytoparasitica 2003-02, Vol.31 (1), p.61-68
Main Authors: Babu, R. Mohan, Sajeena, A., Seetharaman, K., Rangasamy, P., Ebenezer, E. G., Vidhyasekaran, P.
Format: Article
Language:English
Subjects:
Alternaria alternata
Analytical chemistry
Aquatic plants
Chromatography
Floating plants
Fluorescence
Freshwater
High performance liquid chromatography
Liquid chromatography
Pathogens
Quantitation
Toxins
Water hyacinths
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Summary:An improved high performance liquid chromatographic (HPLC) method was developed for the detection and quantitation of host-specific AA-toxin produced byAlternaria alternata, a water hyacinth pathogen. Precolumn derivatization of the toxin witho-phthalaldehyde (OPA) plus mercaptoethanol yielded highly fluorescent products showing well resolved peaks on reverse-phase HPLC. The minimum amounts of AA-toxins detectable by this method were approximately 1 ng. This method seems to offer advantages over conventional techniques, because prechromatographic derivatization of the toxin with OPA is rapid and easy, and fluorescent derivatives permit excellent detection sensitivity. Toxin production in culture filtrates and spore-germinated fluids of the pathogen were analyzed quantitatively using this procedure.
ISSN:0334-2123
1876-7184
DOI:10.1007/BF02979767