Age-dependent impairment of number and angiogenic potential of adipose tissue-derived progenitor cells

Eur J Clin Invest 2011; 41 (2): 126–133 Background  Adipose tissue‐derived stromal cells (ADSCs) are being recognized as a source of stem cells potentially useful for cardiovascular repair. We analysed the abundance and angiogenic activity of adipose tissue‐derived progenitor cells (PCs) in elderly...

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Published in:European journal of clinical investigation 2011-02, Vol.41 (2), p.126-133
Main Authors: Madonna, Rosalinda, Renna, Francesca V., Cellini, Carlo, Cotellese, Roberto, Picardi, Nicola, Francomano, Franco, Innocenti, Paolo, De Caterina, Raffaele
Format: Article
Language:English
Subjects:
Adipose Tissue - metabolism
Adipose tissue-derived stem cells
Age Factors
Aged
Aged, 80 and over
ageing
angiogenesis
atherosclerosis
Atherosclerosis (general aspects, experimental research)
Atherosclerosis - etiology
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Cardiovascular Diseases - etiology
cardiovascular risk factors
Epidemiology
Female
General aspects
Humans
Male
Medical sciences
Middle Aged
Public health. Hygiene
Public health. Hygiene-occupational medicine
Risk Factors
Statistics as Topic
Stem Cells - metabolism
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Summary:Eur J Clin Invest 2011; 41 (2): 126–133 Background  Adipose tissue‐derived stromal cells (ADSCs) are being recognized as a source of stem cells potentially useful for cardiovascular repair. We analysed the abundance and angiogenic activity of adipose tissue‐derived progenitor cells (PCs) in elderly patients most likely to benefit from this novel source of stem cells. Materials and methods  Fifty‐two subjects (aged 68 ± 13 years) with variable degrees of cardiovascular risk underwent abdominal surgery for intercurrent diseases. Visceral adipose tissue (3 ± 1 g visceral fat per patient) was processed with type‐1 collagenase to obtain ADSCs from the stromal‐vascular fraction. Adipose tissue‐derived PCs were quantified by flow cytometry as %CD45−/CD34+/CD133+ cells of total ADSCs. Matrigel angiogenesis assay was used to analyse the ability of ADSCs to form tubes or networks. Results  We found no correlations between number of CD45−/CD34+/CD133+ or total ADSCs and quantitative risk parameters including total cholesterol, low density lipoprotein cholesterol, high density lipoprotein cholesterol, waist circumference, body mass index, and systolic and diastolic arterial pressure. However, increasing age (r = −0·31, P 
ISSN:0014-2972
1365-2362
DOI:10.1111/j.1365-2362.2010.02384.x