Development of species-specific polymerase chain reaction primers for detection of Fusobacterium periodonticum

ABSTRACT The purpose of this study was to develop species‐specific PCR primers for detection of Fusobacterium periodonticum. The specificity data showed that two sets of PCR primers, Fp‐F3/Fp‐R2 and Fp‐F1/Fp‐R2 PCR, produced amplicons from all the F. periodonticum, but not from the other species tes...

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Bibliographic Details
Published in:Microbiology and immunology 2010-12, Vol.54 (12), p.750-753
Main Authors: Park, Soon-Nang, Park, Jae-Yoon, Kook, Joong-Ki
Format: Article
Language:English
Subjects:
5′dUTPase gene
DNA Primers - genetics
Fusobacterium - genetics
Fusobacterium - isolation & purification
Fusobacterium periodonticum
Polymerase Chain Reaction - methods
Species Specificity
species-specific PCR primer
zinc protease gene
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Summary:ABSTRACT The purpose of this study was to develop species‐specific PCR primers for detection of Fusobacterium periodonticum. The specificity data showed that two sets of PCR primers, Fp‐F3/Fp‐R2 and Fp‐F1/Fp‐R2 PCR, produced amplicons from all the F. periodonticum, but not from the other species tested, which included 12 Fusobacterium species or subspecies and representative oral bacteria. The sensitivity of the primer sets was 4 or 40 pg of the chromosomal DNA from F. periodonticum ATCC 33693T. These results suggest that these two sets of PCR primers are quite sensitive in detection of F. periodonticum in molecular epidemiological studies of periodontitis.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.2010.00279.x