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Spurious hypercalcitoninemia and heterophilic antibodies in patients with thyroid nodules
Background The purpose of our study was to examine the prevalence of significant heterophilic antibody (HAb) interferences in serum calcitonin measurement in a large cohort of patients with thyroid nodules. Methods Enrolled in the study were 378 patients with thyroid nodules shown not to have medull...
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| Published in: | Head & neck 2011-01, Vol.33 (1), p.95-97 |
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| container_title | Head & neck |
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| creator | Giovanella, Luca Suriano, Sergio |
| description | Background
The purpose of our study was to examine the prevalence of significant heterophilic antibody (HAb) interferences in serum calcitonin measurement in a large cohort of patients with thyroid nodules.
Methods
Enrolled in the study were 378 patients with thyroid nodules shown not to have medullary thyroid carcinoma (MTC) after extensive diagnostic workup. Serum calcitonin measurement was performed before and after incubating each serum sample in heterophilic‐blocking tubes (HBTs) and the differences were calculated. Samples showing an absolute percentage difference greater than 3 SD from the mean percentage difference were considered as affected by heterophilic antibody interference.
Results
Five of 378 patients (1.3%) with HAb interferences were identified, 4 with clinically relevant false‐positive calcitonin results.
Conclusion
A false‐positive calcitonin result due to HAb interference occurs more frequently than MTC (1.3% vs 0%) in our patient series. A serum pretreatment in HBTs should be considered when increased serum calcitonin levels are found in a patient with a thyroid nodule to prevent unwarranted investigations or therapies. © 2010 Wiley Periodicals, Inc. Head Neck, 2011 |
| doi_str_mv | 10.1002/hed.21405 |
| format | article |
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The purpose of our study was to examine the prevalence of significant heterophilic antibody (HAb) interferences in serum calcitonin measurement in a large cohort of patients with thyroid nodules.
Methods
Enrolled in the study were 378 patients with thyroid nodules shown not to have medullary thyroid carcinoma (MTC) after extensive diagnostic workup. Serum calcitonin measurement was performed before and after incubating each serum sample in heterophilic‐blocking tubes (HBTs) and the differences were calculated. Samples showing an absolute percentage difference greater than 3 SD from the mean percentage difference were considered as affected by heterophilic antibody interference.
Results
Five of 378 patients (1.3%) with HAb interferences were identified, 4 with clinically relevant false‐positive calcitonin results.
Conclusion
A false‐positive calcitonin result due to HAb interference occurs more frequently than MTC (1.3% vs 0%) in our patient series. A serum pretreatment in HBTs should be considered when increased serum calcitonin levels are found in a patient with a thyroid nodule to prevent unwarranted investigations or therapies. © 2010 Wiley Periodicals, Inc. Head Neck, 2011</description><identifier>ISSN: 1043-3074</identifier><identifier>EISSN: 1097-0347</identifier><identifier>DOI: 10.1002/hed.21405</identifier><identifier>PMID: 20848426</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Antibodies, Heterophile - blood ; Antibodies, Heterophile - metabolism ; Biological and medical sciences ; Biomarkers, Tumor - blood ; Biopsy, Needle ; calcitonin ; Calcitonin - blood ; Calcitonin - metabolism ; Carcinoma, Neuroendocrine ; Cohort Studies ; Diagnosis, Differential ; Endocrinopathies ; False Positive Reactions ; Female ; heterophilic antibodies ; Humans ; Immunohistochemistry ; Male ; Malignant tumors ; Medical sciences ; medullary thyroid carcinoma ; Non tumoral diseases. Target tissue resistance. Benign neoplasms ; Otorhinolaryngology. Stomatology ; Sensitivity and Specificity ; Thyroid Neoplasms - blood ; Thyroid Neoplasms - diagnosis ; Thyroid Neoplasms - immunology ; Thyroid Nodule - blood ; Thyroid Nodule - diagnosis ; Thyroid Nodule - immunology ; Thyroid Nodule - surgery ; Thyroid. Thyroid axis (diseases) ; Thyroidectomy - methods</subject><ispartof>Head & neck, 2011-01, Vol.33 (1), p.95-97</ispartof><rights>Copyright © 2010 Wiley Periodicals, Inc.</rights><rights>2015 INIST-CNRS</rights><rights>2010 Wiley Periodicals, Inc. Head Neck, 2011.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3925-c96a639024b7246cfaaab3ea02f2b2f68874e76aabb7d1a0de0ebea45e4339643</citedby><cites>FETCH-LOGICAL-c3925-c96a639024b7246cfaaab3ea02f2b2f68874e76aabb7d1a0de0ebea45e4339643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23794176$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20848426$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Giovanella, Luca</creatorcontrib><creatorcontrib>Suriano, Sergio</creatorcontrib><title>Spurious hypercalcitoninemia and heterophilic antibodies in patients with thyroid nodules</title><title>Head & neck</title><addtitle>Head Neck</addtitle><description>Background
The purpose of our study was to examine the prevalence of significant heterophilic antibody (HAb) interferences in serum calcitonin measurement in a large cohort of patients with thyroid nodules.
Methods
Enrolled in the study were 378 patients with thyroid nodules shown not to have medullary thyroid carcinoma (MTC) after extensive diagnostic workup. Serum calcitonin measurement was performed before and after incubating each serum sample in heterophilic‐blocking tubes (HBTs) and the differences were calculated. Samples showing an absolute percentage difference greater than 3 SD from the mean percentage difference were considered as affected by heterophilic antibody interference.
Results
Five of 378 patients (1.3%) with HAb interferences were identified, 4 with clinically relevant false‐positive calcitonin results.
Conclusion
A false‐positive calcitonin result due to HAb interference occurs more frequently than MTC (1.3% vs 0%) in our patient series. A serum pretreatment in HBTs should be considered when increased serum calcitonin levels are found in a patient with a thyroid nodule to prevent unwarranted investigations or therapies. © 2010 Wiley Periodicals, Inc. Head Neck, 2011</description><subject>Antibodies, Heterophile - blood</subject><subject>Antibodies, Heterophile - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - blood</subject><subject>Biopsy, Needle</subject><subject>calcitonin</subject><subject>Calcitonin - blood</subject><subject>Calcitonin - metabolism</subject><subject>Carcinoma, Neuroendocrine</subject><subject>Cohort Studies</subject><subject>Diagnosis, Differential</subject><subject>Endocrinopathies</subject><subject>False Positive Reactions</subject><subject>Female</subject><subject>heterophilic antibodies</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Malignant tumors</subject><subject>Medical sciences</subject><subject>medullary thyroid carcinoma</subject><subject>Non tumoral diseases. Target tissue resistance. Benign neoplasms</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Sensitivity and Specificity</subject><subject>Thyroid Neoplasms - blood</subject><subject>Thyroid Neoplasms - diagnosis</subject><subject>Thyroid Neoplasms - immunology</subject><subject>Thyroid Nodule - blood</subject><subject>Thyroid Nodule - diagnosis</subject><subject>Thyroid Nodule - immunology</subject><subject>Thyroid Nodule - surgery</subject><subject>Thyroid. Thyroid axis (diseases)</subject><subject>Thyroidectomy - methods</subject><issn>1043-3074</issn><issn>1097-0347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp1kE1v1DAQhq0K1C966B9AuSDEIe34I3ZyREvbRapAakG0J8txJoohm6S2o7L_vl52F06cZvTqmRnNQ8g5hQsKwC47bC4YFVAckGMKlcqBC_Vq0wuec1DiiJyE8BMAuBTskBwxKEUpmDwmj_fT7N04h6xbT-it6a2L4-AGXDmTmaHJOozox6lzvbMpiK4eG4chc0M2mehwiCF7drHLYrf2o2uyYWzmHsMb8ro1fcCzXT0l36-vvi2W-e3Xm8-Lj7e55RUrcltJI3kFTNSKCWlbY0zN0QBrWc1aWZZKoJIprFVDDTQIWKMRBQrOKyn4KXm_3Tv58WnGEPXKBYt9bwZMf-mSV4oWjNNEftiS1o8heGz15N3K-LWmoDcidRKp_4hM7Nvd1rlepXRP7s0l4N0OMCFZa70ZrAv_OK4qQdWGu9xyz67H9f8v6uXVp_3pfDvhQsTffyeM_6Wl4qrQP77c6OUCHuBa3mnFXwDU9Jpr</recordid><startdate>201101</startdate><enddate>201101</enddate><creator>Giovanella, Luca</creator><creator>Suriano, Sergio</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201101</creationdate><title>Spurious hypercalcitoninemia and heterophilic antibodies in patients with thyroid nodules</title><author>Giovanella, Luca ; Suriano, Sergio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3925-c96a639024b7246cfaaab3ea02f2b2f68874e76aabb7d1a0de0ebea45e4339643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Antibodies, Heterophile - blood</topic><topic>Antibodies, Heterophile - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - blood</topic><topic>Biopsy, Needle</topic><topic>calcitonin</topic><topic>Calcitonin - blood</topic><topic>Calcitonin - metabolism</topic><topic>Carcinoma, Neuroendocrine</topic><topic>Cohort Studies</topic><topic>Diagnosis, Differential</topic><topic>Endocrinopathies</topic><topic>False Positive Reactions</topic><topic>Female</topic><topic>heterophilic antibodies</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Malignant tumors</topic><topic>Medical sciences</topic><topic>medullary thyroid carcinoma</topic><topic>Non tumoral diseases. Target tissue resistance. Benign neoplasms</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Sensitivity and Specificity</topic><topic>Thyroid Neoplasms - blood</topic><topic>Thyroid Neoplasms - diagnosis</topic><topic>Thyroid Neoplasms - immunology</topic><topic>Thyroid Nodule - blood</topic><topic>Thyroid Nodule - diagnosis</topic><topic>Thyroid Nodule - immunology</topic><topic>Thyroid Nodule - surgery</topic><topic>Thyroid. Thyroid axis (diseases)</topic><topic>Thyroidectomy - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Giovanella, Luca</creatorcontrib><creatorcontrib>Suriano, Sergio</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Head & neck</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Giovanella, Luca</au><au>Suriano, Sergio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spurious hypercalcitoninemia and heterophilic antibodies in patients with thyroid nodules</atitle><jtitle>Head & neck</jtitle><addtitle>Head Neck</addtitle><date>2011-01</date><risdate>2011</risdate><volume>33</volume><issue>1</issue><spage>95</spage><epage>97</epage><pages>95-97</pages><issn>1043-3074</issn><eissn>1097-0347</eissn><abstract>Background
The purpose of our study was to examine the prevalence of significant heterophilic antibody (HAb) interferences in serum calcitonin measurement in a large cohort of patients with thyroid nodules.
Methods
Enrolled in the study were 378 patients with thyroid nodules shown not to have medullary thyroid carcinoma (MTC) after extensive diagnostic workup. Serum calcitonin measurement was performed before and after incubating each serum sample in heterophilic‐blocking tubes (HBTs) and the differences were calculated. Samples showing an absolute percentage difference greater than 3 SD from the mean percentage difference were considered as affected by heterophilic antibody interference.
Results
Five of 378 patients (1.3%) with HAb interferences were identified, 4 with clinically relevant false‐positive calcitonin results.
Conclusion
A false‐positive calcitonin result due to HAb interference occurs more frequently than MTC (1.3% vs 0%) in our patient series. A serum pretreatment in HBTs should be considered when increased serum calcitonin levels are found in a patient with a thyroid nodule to prevent unwarranted investigations or therapies. © 2010 Wiley Periodicals, Inc. Head Neck, 2011</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>20848426</pmid><doi>10.1002/hed.21405</doi><tpages>3</tpages></addata></record> |
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| subjects | Antibodies, Heterophile - blood Antibodies, Heterophile - metabolism Biological and medical sciences Biomarkers, Tumor - blood Biopsy, Needle calcitonin Calcitonin - blood Calcitonin - metabolism Carcinoma, Neuroendocrine Cohort Studies Diagnosis, Differential Endocrinopathies False Positive Reactions Female heterophilic antibodies Humans Immunohistochemistry Male Malignant tumors Medical sciences medullary thyroid carcinoma Non tumoral diseases. Target tissue resistance. Benign neoplasms Otorhinolaryngology. Stomatology Sensitivity and Specificity Thyroid Neoplasms - blood Thyroid Neoplasms - diagnosis Thyroid Neoplasms - immunology Thyroid Nodule - blood Thyroid Nodule - diagnosis Thyroid Nodule - immunology Thyroid Nodule - surgery Thyroid. Thyroid axis (diseases) Thyroidectomy - methods |
| title | Spurious hypercalcitoninemia and heterophilic antibodies in patients with thyroid nodules |
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