Canine urinary bladder epithelial cells: preparation for cell culture by enzyme dispersion

In a qualitative and quantitative study of enzymic dispersion of cells from the mucosal layer stripped from canine urinary bladder, trypsin was found to be equal or superior to the other enzymes tested for dispersal of urothelial cells specifically. Collagenase or collagenase plus trypsin served to...

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Bibliographic Details
Published in:Urological research 1977-01, Vol.5 (2), p.87-94
Main Authors: Bonar, R A, Reich, 3rd, C F, Sharief, Y
Format: Article
Language:English
Subjects:
Animals
Culture Techniques
Dogs
Epithelial Cells
Epithelium - enzymology
Microbial Collagenase - metabolism
Trypsin - metabolism
Urinary Bladder - enzymology
Urinary Bladder - pathology
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Summary:In a qualitative and quantitative study of enzymic dispersion of cells from the mucosal layer stripped from canine urinary bladder, trypsin was found to be equal or superior to the other enzymes tested for dispersal of urothelial cells specifically. Collagenase or collagenase plus trypsin served to disperse the whole tissue. A procedure for recovering the urothelial cells as a single-cell suspension and establishing them in culture is presented. The morphology, culture behaviour, and chromosome complement of these cells is described.
ISSN:0300-5623
1434-0879
DOI:10.1007/BF00256846