Cell Size in Aging Monolayer Cultures

Changes in the size of the area covered by individual cultured WI-38 cells as the cultures age have been studied by using a new microphotographic paper cutout technique. This method is nondestructive and nonintrusive and avoids a number of artifacts which can occur in the measurement of suspended ce...

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Bibliographic Details
Published in:In Vitro 1977-05, Vol.13 (5), p.297-300
Main Authors: S. B. Greenberg, G. L. Grove, Cristofalo, V. J.
Format: Article
Language:English
Subjects:
Animal cells
Cell culture techniques
Cell Division
Cell growth
Cell Line
Cell lines
Cell Survival
Cells
Cells, Cultured - cytology
Cellular differentiation
Cellular senescence
Cultured cells
Cytological Techniques
Diploidy
Fibroblasts
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Summary:Changes in the size of the area covered by individual cultured WI-38 cells as the cultures age have been studied by using a new microphotographic paper cutout technique. This method is nondestructive and nonintrusive and avoids a number of artifacts which can occur in the measurement of suspended cells. The measurements reveal that the decreased cell yield of late passage cultures reflects not only the appearance of a subpopulation of larger cells but also the failure of the cells to utilize all the growth surface available to them.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02616174