N,N-Dimethylformamide-induced morphological differentiation and reduction of tumorigenicity in cultured mouse rhabdomyosarcoma cells

N,N-Dimethylformamide treatment of cell cultures established from a transplantable murine rhabdomyosarcoma-induced morphological differentiation and a marked reduction in the tumorigenicity of the sarcoma cells. Fourteen of 17 CE/J mice receiving injections of inducer-treated cells did not develop t...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1977-09, Vol.37 (9), p.3136-3140
Main Author: Dexter, D L
Format: Article
Language:English
Subjects:
Animals
Bucladesine - pharmacology
Cell Differentiation
Cell Division - drug effects
Cell Survival - drug effects
Cells, Cultured
Dimethylformamide - pharmacology
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
Rhabdomyosarcoma - drug therapy
Rhabdomyosarcoma - pathology
Sarcoma, Experimental - drug therapy
Sarcoma, Experimental - pathology
Transplantation, Homologous
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Summary:N,N-Dimethylformamide treatment of cell cultures established from a transplantable murine rhabdomyosarcoma-induced morphological differentiation and a marked reduction in the tumorigenicity of the sarcoma cells. Fourteen of 17 CE/J mice receiving injections of inducer-treated cells did not develop tumors after 6 months, whereas all 21 mice receiving inocula of untreated sarcoma cells died of disease between 11 and 31 days. The drug-treated cells did not grow in soft agar; untreated tumor cells grew in the semisolid medium. The untreated tumor cells showed a reduced serum requirement and had a higher saturation density compared to drug-treated cells. Thus the reduction in tumorigenicity of N,N-dimethylformamide-treated cells correlated with certain in vitro growth properties that are more characteristic of normal, mesenchymally derived cells than of sarcoma cells.
ISSN:0008-5472