Acetylcholine and cAMP in adrenal medulla:indirect effect

Catecholamine release and cAMP accumulation were studied in bovine adrenal medulla slices in vitro. Acetylcholine (10(-4) M) and salbutamol (10(-6) M) caused increased release of catecholamines and accumulation of cAMP. Incubation in Ca2+ -free medium abolished the release of catecholamines and the...

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Bibliographic Details
Published in:Naunyn-Schmiedeberg's archives of pharmacology 1977-04, Vol.297 (3), p.241-243
Main Authors: Boonyaviroj, P, Gutman, Y
Format: Article
Language:English
Subjects:
Acetylcholine - pharmacology
Adenylyl Cyclases - metabolism
Adrenal Medulla - drug effects
Adrenal Medulla - enzymology
Adrenal Medulla - metabolism
Albuterol - pharmacology
Animals
Calcium - pharmacology
Catecholamines - metabolism
Cattle
Cyclic AMP - metabolism
In Vitro Techniques
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Summary:Catecholamine release and cAMP accumulation were studied in bovine adrenal medulla slices in vitro. Acetylcholine (10(-4) M) and salbutamol (10(-6) M) caused increased release of catecholamines and accumulation of cAMP. Incubation in Ca2+ -free medium abolished the release of catecholamines and the increase of cAMP caused by acetylcholine but not that caused by salbutamol. In a membrane fraction of adrenal medulla acetylcholine (10(-4) M) had no effect on adenylate cyclase activity but salbutamol (10(-6) M) caused substantial activation of adenylate cyclase. It is suggested that acetylcholine has no direct effect on adenylate cyclase or cAMP in adrenal medulla and the accumulation of cAMP observed in slices incubated with acetylcholine is due to the effect of catecholamines, released by acetylcholine, on the medullary cells.
ISSN:0028-1298
1432-1912
DOI:10.1007/bf00509267