Use of Ficoll-sodium metrizoate density gradient to separate human X- and Y-bearing spermatozoa

SEVERAL claims have been made about the successful separation of Y-bearing spermatozoa in the past 1 . The most recent claim by Ericsson et al. 2 has been disputed 3,4 . We have attempted to develop a method for separating human X- and Y-bearing spermatozoa, without affecting the motility or the var...

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Bibliographic Details
Published in:Nature (London) 1977-09, Vol.269 (5623), p.58-60
Main Authors: SHASTRY, PADMA R, HEGDE, UMASHASHI C, RAO, SHANTA S
Format: Article
Language:English
Subjects:
Cell Separation - methods
Cell Survival
Centrifugation, Density Gradient
Ficoll
Genetic Engineering
Humanities and Social Sciences
Humans
letter
Male
Metrizoic Acid
multidisciplinary
Science
Sex Chromosomes
Sex Preselection
Sperm Motility
Spermatozoa
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Summary:SEVERAL claims have been made about the successful separation of Y-bearing spermatozoa in the past 1 . The most recent claim by Ericsson et al. 2 has been disputed 3,4 . We have attempted to develop a method for separating human X- and Y-bearing spermatozoa, without affecting the motility or the variability of these vital cells. We report here a successful separation of human X- and Y-bearing spermatozoa using a two-step procedure with Ficoll–Sodium metrizoate density gradient. The motility and viability of enriched fractions of spermatozoa was found to be slightly reduced. The reduction was mainly due to centrifugation and not the density gradient. The ability to predetermine the sex of the offspring before conception would have great clinical and sociological significance 5 .
ISSN:0028-0836
1476-4687
DOI:10.1038/269058a0