Cytomat-R: a computer-controlled multiple laser source multiparameter flow cytophotometer system

A multiple illumination wavelength multiparameter flow cytophotometer system, using laser sources and controlled by a small, general-purpose digital computer, has been produced for use in the development of new flow cytometric techniques. Three different laser wave-lengths can be used simultaneously...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1977-07, Vol.25 (7), p.836-844
Main Authors: Shapiro, HM, Schildkraut, ER, Curbelo, R, Turner, RB, Webb, RH, Brown, DC, Block, MJ
Format: Article
Language:English
Subjects:
Blood Cells
Cell Count - instrumentation
Computers
Cytological Techniques - instrumentation
Lasers
Spectrometry, Fluorescence - instrumentation
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Description
Summary:A multiple illumination wavelength multiparameter flow cytophotometer system, using laser sources and controlled by a small, general-purpose digital computer, has been produced for use in the development of new flow cytometric techniques. Three different laser wave-lengths can be used simultaneously to illuminate different regions of the flow chamber; as many as five measurements of light scattering at various angles, extinction, and fluorescence at one or more wavelengths can be made at each illuminated station. Cells in suspension may be examined at rates of 1000 cells/sec, with seven correlated optical measurements being recorded for each cell. A library of programs for data manipulation and statistical analysis make it possible to use the system to develop and implement cell characterization, counting and classification procedures for basic and clinical research applications.
ISSN:0022-1554
1551-5044
DOI:10.1177/25.7.330733