An Improved Organ Culture Method for Adult Mammalian Lung

An improved method for maintaining adult rat lung in submerged organ culture is described in which the alveoli were inflated with agar and 200-µm-thick hand-cut sections were mounted in Rose chambers. The conventional single-compartmented Rose culture chamber was modified by adding a second chamber...

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Bibliographic Details
Published in:In Vitro 1977-08, Vol.13 (8), p.517-524
Main Authors: Robert R. Guerrero, Rounds, Donald E., Booher, Jon
Format: Article
Language:English
Subjects:
Animals
Body tissues
Catheterization
Cell Survival
Culture Media
Dialysis
Epithelial cells
Gaskets
Lung
Lungs
Organ culture techniques
Organ Culture Techniques - instrumentation
Organ Culture Techniques - methods
Oxygen
Pulmonary alveoli
Rats
Tissue culture techniques
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Summary:An improved method for maintaining adult rat lung in submerged organ culture is described in which the alveoli were inflated with agar and 200-µm-thick hand-cut sections were mounted in Rose chambers. The conventional single-compartmented Rose culture chamber was modified by adding a second chamber separated from the first by a gaspermeable membrane. One compartment functioned as an air reservoir and the other housed the explants submerged in nutrient medium. Visking dialysis membrane used underneath the explants prevented cell outgrowth and facilitated the exchange of nutrients and waste products at the glass-tissue interface. Because of the excellent optical properties of the Rose chamber and the thinness of the explants, individual cell types can be identified in the living tissue. The explants were studied with time-lapse cinematography, light microscopy, histology, and with erythrosine B for dye exclusion. With this modified system the functional life span of the explants was increased from 1 week to 1 month.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02615145