Purification of Human Angiotensinogen

SUMMARY A four-step method for the purification of human angiotensinogen has been devised. The four steps are(1) removal of albumin by affinity chromatography on blue dextran-Sepharose, (2) chromatography on DEAESephadex, (3) chromatography on hydroxylapatite, and (4) chromatography on DEAE-cellulos...

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Bibliographic Details
Published in:Circulation research 1977-10, Vol.41 (4 Suppl II), p.II-29-33-29-33
Main Authors: TEWKSBURY, D A, PREMEAU, M R, DUMAS, M L, FROME, W L
Format: Article
Language:English
Subjects:
Angiotensinogen - isolation & purification
Angiotensins - isolation & purification
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Humans
Immunoelectrophoresis
Methods
Ultracentrifugation
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Summary:SUMMARY A four-step method for the purification of human angiotensinogen has been devised. The four steps are(1) removal of albumin by affinity chromatography on blue dextran-Sepharose, (2) chromatography on DEAESephadex, (3) chromatography on hydroxylapatite, and (4) chromatography on DEAE-cellulose. This method is capable of producing 8 mg of purified angiotensinogen from 150 ml of plasma with 33% overall recovery of reninreleasable angiotensin I. The angiotensinogen appears homogeneous by immunochemical and ultracentrifugal techniques. The N-terminal amino acids have been determined to be alanine and aspartic acid or asparagine.
ISSN:0009-7330
1524-4571
DOI:10.1161/01.res.41.4.29