Binding of colonic tissue membrane to mononuclear peripheral blood leukocytes

Colonic tissue membrane binding to peripheral blood mononuclear leukocytes was quantitated by 125I labeling of membrane fragments and by determining the acquisition of membrane-specific enzyme activity and radioactivity in mononuclear cells after contact with the tissue membrane fragments. Mononucle...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1977-10, Vol.37 (10), p.3711-3719
Main Author: Wolberg, W H
Format: Article
Language:English
Subjects:
Adenosine Monophosphate - metabolism
Adenosine Triphosphate - metabolism
Alkaline Phosphatase - metabolism
Antigens, Neoplasm
B-Lymphocytes - metabolism
Binding Sites
Cell Membrane - immunology
Cell Membrane - metabolism
Colon - immunology
Colon - metabolism
Colonic Neoplasms - immunology
Colonic Neoplasms - metabolism
Humans
Hypoxanthines - metabolism
Immunity, Cellular
In Vitro Techniques
Inosine - metabolism
Leukocytes - immunology
Leukocytes - metabolism
Levamisole - pharmacology
T-Lymphocytes - metabolism
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Summary:Colonic tissue membrane binding to peripheral blood mononuclear leukocytes was quantitated by 125I labeling of membrane fragments and by determining the acquisition of membrane-specific enzyme activity and radioactivity in mononuclear cells after contact with the tissue membrane fragments. Mononuclear cells bound equal amounts of normal and tumor tissue membrane fragments. Mononuclear cells capable of binding homologous but not autologous colonic tissue membranes were recovered from the peripheral blood of colon cancer-bearing patients. Mononuclear cells capable of binding autologous colonic tissue membranes appeared in the peripheral blood of patients after curative but not palliative tumor resection. Tumor membrane enzymes, including alkaline phosphatase, were introduced to mononuclear cells by bound tissue fragments. The activity of alkaline phosphatase present in the bound membrane fragments was inhibited by the immunorestorative drug, levamisole. Cellular debris liberated from tumors may play an important role in overcoming the host's defenses by binding to mononuclear cells, saturating antigen-binding sites, and introducing exogenous enzymes.
ISSN:0008-5472