Detection of Antibody to Rubella Virus by Enzyme-Linked Immunosorbent Assay

Titers of antibody to rubella virus in 68 human sera were compared by hemagglutination inhibition and enzyme-linked immunosorbent assay (ELISA). In general, the titers measured by ELISA were higher than those found by hemagglutination inhibition. Although the titers differed, the two methods showed...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1977-10, Vol.136 (Supplement-2), p.S300-S303
Main Authors: Gravell, Maneth, Dorsett, Preston H., Gutenson, Otto, Ley, Anita C.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Viral - isolation & purification
Antigens
Enzyme linked immunosorbent assay
False Positive Reactions
Hemagglutination
Hemagglutination Inhibition Tests
Humans
Infections
Infectious diseases
Rabbits
Rubella
Rubella virus
Rubella virus - immunology
Scientific Articles
Vero cells
Viruses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Titers of antibody to rubella virus in 68 human sera were compared by hemagglutination inhibition and enzyme-linked immunosorbent assay (ELISA). In general, the titers measured by ELISA were higher than those found by hemagglutination inhibition. Although the titers differed, the two methods showed parallel trends. Use of purified rubella virus and addition of 1% bovine serum albumin to the test wash solution aided in reducing false-positive titers in ELISA.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/136.Supplement_2.S300