The Rapid Restoration of Respiratory Control to Uncoupled Mitochondria

A polarographic technique was used to demonstrate the rapid restoration by bovine serum albumin of respiratory control to rat liver mitochondria uncoupled with various reagents. Albumin also restored respiratory control to mitochondria isolated from animals treated by injection with an uncoupling re...

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Bibliographic Details
Published in:The Journal of biological chemistry 1966-08, Vol.241 (16), p.3708-3713
Main Authors: Weinbach, E C, Garbus, J
Format: Article
Language:English
Subjects:
Animals
Benzimidazoles - pharmacology
Dinitrophenols - pharmacology
Glutamates - pharmacology
Hydroxybutyrates - pharmacology
Liver - cytology
Mitochondria - metabolism
Oxygen Consumption
Phenols - pharmacology
Polarography
Rats
Serum Albumin, Bovine - pharmacology
Succinates - pharmacology
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Summary:A polarographic technique was used to demonstrate the rapid restoration by bovine serum albumin of respiratory control to rat liver mitochondria uncoupled with various reagents. Albumin also restored respiratory control to mitochondria isolated from animals treated by injection with an uncoupling reagent and to mitochondria incubated with the reagent prior to the polarographic assay. Albumin was effective with the nitro- and halo-substituted phenols, thyroxine, Dicumarol, carbonyl cyanide m -chlorophenylhydrazone, and 4,5,6,7-tetrachloro-2-trifluoromethylbenzimidazole. Albumin was ineffective in counteracting the inhibitory action of gramicidin, antimycin A, oligomycin, and rotenone. Although the addition of an amount of albumin equimolar to that of the uncoupling reagent present had a substantial effect, amounts of the protein at least twice equimolar were required for the rapid and essentially complete restoration of respiratory control with most of the reagents tested.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)99830-3