The Amino Acid Sequence of Bovine Heart Cytochrome c

The amino acid composition of beef heart cytochrome c was determined. The protein was digested with chymotrypsin and trypsin and the digests were resolved by ion exchange chromatography on Dowex 50 with volatile buffers. From the chymotryptic and tryptic digests, 16 and 17 fractions, respectively, w...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1966-03, Vol.241 (5), p.1166-1177
Main Authors: Nakashima, T, Higa, H, Matsubara, H, Benson, A M, Yasunobu, K T
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cattle
Chromatography, Ion Exchange
Chymotrypsin
Cytochromes
In Vitro Techniques
Myocardium
Peptides
Trypsin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The amino acid composition of beef heart cytochrome c was determined. The protein was digested with chymotrypsin and trypsin and the digests were resolved by ion exchange chromatography on Dowex 50 with volatile buffers. From the chymotryptic and tryptic digests, 16 and 17 fractions, respectively, were isolated and characterized. From the overlapping composition and sequences plus the use of the chemical reagents, cyanogen bromide and N -bromosuccinimide, the complete sequence of 104 residues was deduced. Bovine heart cytochrome c has an amino acid sequence identical with that of pig and sheep cytochromes c .
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)96817-1