Disk Electrophoresis of Plasmodium berghei

A method of analytic separation of soluble proteins in aqueous extracts of Plasmodium berghei is described. Extracts are prepared by sonication of suspensions of whole washed parasites obtained by saponin lysis of infected rat red cells. Separation of the extracts into reproducible electrophoretic p...

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Bibliographic Details
Published in:The Journal of parasitology 1966-02, Vol.52 (1), p.23-25
Main Authors: Sodeman, William A., Joseph H. E. T. Meuwissen
Format: Article
Language:English
Subjects:
Animals
Antigens
Centrifugation
Chemical suspensions
Electrophoresis
Gels
In Vitro Techniques
Malaria
Parasite hosts
Parasites
Plasmodium
Proteins
Rats
Saponins
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Description
Summary:A method of analytic separation of soluble proteins in aqueous extracts of Plasmodium berghei is described. Extracts are prepared by sonication of suspensions of whole washed parasites obtained by saponin lysis of infected rat red cells. Separation of the extracts into reproducible electrophoretic patterns is carried out by zone electrophoresis on polyacrylamide gel. Extracts prepared in this manner contain a heterogeneous mixture of soluble protein constituents of both host and parasite origin. A minimum of 15 parasite proteins and an additional minimum of six proteins of host origin have been identified.
ISSN:0022-3395
1937-2345
DOI:10.2307/3276385