HISTOCHEMICAL AND ULTRASTRUCTURAL DEMONSTRATION OF γ-GLUTAMYL TRANSPEPTIDASE ACTIVITY

A simultaneous coupling azo dye method for the histochemical demonstration of γ-glutamyl transpeptidase activity using the new substrate γ-glutamyl-4-methoxy-2-naphthylamide has been described. The method appears superior to previously reported methods for γ-glutamyl transpeptidase activity and can...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1969-08, Vol.17 (8), p.517-526
Main Authors: RUTENBURG, ALEXANDER M., KIM, HWAKYU, FISCHBEIN, JEROME W., HANKER, JACOB S., WASSERKRUG, HANNAH L., SELIGMAN, ARNOLD M.
Format: Article
Language:English
Subjects:
Acyltransferases - analysis
Amides
Animals
Azides
Azo Compounds
Brain - enzymology
Capillaries - enzymology
Copper
Diazonium Compounds
Digestive System - enzymology
Female
Genitalia, Male - enzymology
Glutamates
Histocytochemistry
Kidney - enzymology
Liver - enzymology
Lung - enzymology
Male
Methods
Microscopy, Electron
Naphthalenes
Osmium
Ovary - enzymology
Pancreas - enzymology
Rats
Spleen - enzymology
Thymus Gland - enzymology
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Summary:A simultaneous coupling azo dye method for the histochemical demonstration of γ-glutamyl transpeptidase activity using the new substrate γ-glutamyl-4-methoxy-2-naphthylamide has been described. The method appears superior to previously reported methods for γ-glutamyl transpeptidase activity and can easily be modified for the electron microscopic localization of the enzyme by bridging osmium to the copper chelate of the azo dye via thiocarbohydrazide. The optimum conditions for the histochemical reaction were developed and the distribution of enzymatic activity in the tissues of the rat is described for light microscopy and with rat pancreas for electron microscopy. The electron-opaque deposits were seen in the endoplasmic reticulum in the vicinity of the zymogen granules in the apical portion of the acinar cell.
ISSN:0022-1554
1551-5044
DOI:10.1177/17.8.517