A NEW ION ASSOCIATION TECHNIQUE FOR DEMONSTRATING POLYANIONS IN TISSUE SECTIONS

A new ion association technique was developed in our laboratory to demonstrate polyanions histochemically. The identification of the polyanions was effected in two steps: (1) the formation of a polyanion-quaternary ammonium complex following treatment with a quaternary salt and (2) subsequent format...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1966-02, Vol.14 (2), p.147-152
Main Authors: ZUGIBE, FREDERICK T, FINK, MARILYN L
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents
Aorta
Cartilage
Coronary Vessels
Dogs
Flavobacterium
Histocytochemistry
Humans
Hyaluronoglucosaminidase
In Vitro Techniques
Ions
Iron
Male
Pancreas
Parotid Gland
Testis - enzymology
Thiocyanates
Umbilical Cord
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Summary:A new ion association technique was developed in our laboratory to demonstrate polyanions histochemically. The identification of the polyanions was effected in two steps: (1) the formation of a polyanion-quaternary ammonium complex following treatment with a quaternary salt and (2) subsequent formation of a red ion association complex with the quaternary moiety following treatment with ferric thiocyanate solution. Chemical studies indicated that the ion association complexes are cetyltrimethylammonium-hexathiocyanatoferrate (iii) and cetylpyridinium-hexathiocyanatoferrate (iii). These are red in color, very soluble in organic solvents such as chloroform, ethanol, methanol, acetone and xylene and insoluble in water.
ISSN:0022-1554
1551-5044
DOI:10.1177/14.2.147