Determination of Dihydroseselins in Fruits and Extracts of Ammi visnaga L

Three methods for the assay of dihydroseselins in the fruits and extracts of Ammi visnaga are proposed.The first is based on measuring the absorption maximum of the dihydroseselins at 323 mu, with a sensitivity range of 2-20 mcg./ml.The second and the third are colorimetric in which visnadin was cle...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1969-12, Vol.58 (12), p.1545-1547
Main Authors: Karawya, M.S., Sina, A., Nour, G.
Format: Article
Language:English
Subjects:
Ammi visnuga
Ammi visnuga, fruits and extracts—assay of dihydroseselins
assay
botany
Chromatography, Thin Layer
Coumarins - analysis
Dihydroseselins
Dihydroseselins, assay
fruits and extracts-assay of dihydroseselins
identification
Methods
Plant Extracts - analysis
Plant Science and Plant Products
plants
Plants - analysis
Spectrophotometry
TLC-separation
TLC—separation, identification
UV spectrophotometry-analysis
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Summary:Three methods for the assay of dihydroseselins in the fruits and extracts of Ammi visnaga are proposed.The first is based on measuring the absorption maximum of the dihydroseselins at 323 mu, with a sensitivity range of 2-20 mcg./ml.The second and the third are colorimetric in which visnadin was cleaved by cold ethanolic sodium hydroxide and then either coupled with the diazol solution or reacted with 2,6-dibromoquinone chlorimide.The intensity of the resulting colors was then measured spectrophoto-metrically at 500 and 630 mp, respectively, with a sensitivity range of 1-12 mcg./ml.in both methods.The dihydroseselins were separated from Ammi uisnuga by TLC, quantitatively eluted, and comparatively assayed by the above methods.The results were concordant.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600581230